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Anti-ASS1 Antibody Picoband™ (monoclonal, 7I9)

     
  •  - Anti-ASS1 Antibody Picoband™ (monoclonal, 7I9) ABO14985
    Figure 1. Western blot analysis of ASS1 using anti-ASS1 antibody (M02212-2).
    Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
    Lane 1: human Hela whole cell lysates,
    Lane 2: human HEPG2 whole cell lysates,
    Lane 3: human Hek293 wohle cell lysates,
    Lane 4: human THP-1 whole cell lysates,
    Lane 5: human T47D whole cell lysates,
    Lane 6: monkey kidney tissue lysates,
    Lane 7: rat liver tissue lysates,
    Lane 8: rat kidney tissue lysates,
    Lane 9: mouse liver tissue lysates,
    Lane 10: mouse kidney tissue lysates.
    After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-ASS1 antigen affinity purified monoclonal antibody (Catalog # M02212-2) at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for ASS1 at approximately 47KD. The expected band size for ASS1 is at 47KD.
    detail
  •  - Anti-ASS1 Antibody Picoband™ (monoclonal, 7I9) ABO14985
    Figure 2. IHC analysis of ASS1 using anti-ASS1 antibody (M02212-2).
    ASS1 was detected in paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 µg/ml mouse anti-ASS1 Antibody (M02212-2) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.
    detail
  •  - Anti-ASS1 Antibody Picoband™ (monoclonal, 7I9) ABO14985
    Figure 3. IHC analysis of ASS1 using anti-ASS1 antibody (M02212-2).
    ASS1 was detected in paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 µg/ml mouse anti-ASS1 Antibody (M02212-2) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.
    detail
  •  - Anti-ASS1 Antibody Picoband™ (monoclonal, 7I9) ABO14985
    Figure 4. IHC analysis of ASS1 using anti-ASS1 antibody (M02212-2).
    ASS1 was detected in paraffin-embedded section of human prostate cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 µg/ml mouse anti-ASS1 Antibody (M02212-2) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.
    detail
  •  - Anti-ASS1 Antibody Picoband™ (monoclonal, 7I9) ABO14985
    Figure 5. IHC analysis of ASS1 using anti-ASS1 antibody (M02212-2).
    ASS1 was detected in paraffin-embedded section of human colon cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 µg/ml mouse anti-ASS1 Antibody (M02212-2) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.
    detail
  •  - Anti-ASS1 Antibody Picoband™ (monoclonal, 7I9) ABO14985
    Figure 6. IHC analysis of ASS1 using anti-ASS1 antibody (M02212-2).
    ASS1 was detected in paraffin-embedded section of rat liver tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 µg/ml mouse anti-ASS1 Antibody (M02212-2) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.
    detail
  •  - Anti-ASS1 Antibody Picoband™ (monoclonal, 7I9) ABO14985
    Figure 7. IF analysis of ASS1 using anti-ASS1 antibody (M02212-2).
    ASS1 was detected in immunocytochemical section of MCF-7 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 µg/mL mouse anti-ASS1 Antibody (M02212-2) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Mouse IgG (BA1126) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
    detail
  •  - Anti-ASS1 Antibody Picoband™ (monoclonal, 7I9) ABO14985
    Figure 8. Flow Cytometry analysis of SiHa cells using anti-ASS1 antibody (M02212-2).
    Overlay histogram showing SiHa cells stained with M02212-2 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-ASS1 Antibody (M02212-2, 1 µg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-mouse IgG (BA1126, 5-10 µg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse IgG (1 µg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, IHC, IF, ICC, FC
Primary Accession P00966
Host Mouse
Isotype Mouse IgG2b
Reactivity Rat, Human, Mouse, Monkey
Clonality Monoclonal
Format Lyophilized
Description Anti-ASS1 Antibody Picoband™ (monoclonal, 7I9) . Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Monkey, Mouse, Rat.
Reconstitution Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Additional Information
Gene ID 445
Other Names Argininosuccinate synthase, 6.3.4.5, Citrulline--aspartate ligase, ASS1 (HGNC:758)
Calculated MW 47 kDa
Application Details Western blot, 0.25-0.5 µg/ml, Human, Mouse, Rat, Monkey
Immunohistochemistry (Paraffin-embedded Section), 2-5 µg/ml, Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 5 µg/ml, Human
Flow Cytometry, 1-3 µg/1x10^6 cells, Human
Contents Each vial contains 4mg Trehalose, 0.9mg NaCl and 0.2mg Na2HPO4.
Clone Names Clone: 7I9
Immunogen E.coli-derived human ASS1 recombinant protein (Position: S3-S365).
Purification Immunogen affinity purified.
Storage Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Protein Information
Name ASS1 (HGNC:758)
Function One of the enzymes of the urea cycle, the metabolic pathway transforming neurotoxic amonia produced by protein catabolism into inocuous urea in the liver of ureotelic animals. Catalyzes the formation of arginosuccinate from aspartate, citrulline and ATP and together with ASL it is responsible for the biosynthesis of arginine in most body tissues.
Cellular Location Cytoplasm, cytosol
Tissue Location Expressed in adult liver.
Research Areas
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Background

Argininosuccinate synthetase is an enzyme that in humans is encoded by the ASS1 gene. It is mapped to 9q34.11. The protein encoded by this gene catalyzes the penultimate step of the arginine biosynthetic pathway. There are approximately 10 to 14 copies of this gene including the pseudogenes scattered across the human genome, among which the one located on chromosome 9 appears to be the only functional gene for argininosuccinate synthetase. Mutations in the chromosome 9 copy of this gene cause citrullinemia. Two transcript variants encoding the same protein have been found for this gene.

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$ 370.00
Cat# ABO14985
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