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Anti-Actin/ACTA1 Antibody Picoband™ (monoclonal, 3H5)
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| WB, IHC |
|---|---|
| Primary Accession | P68133 |
| Host | Mouse |
| Isotype | Mouse IgG1 |
| Reactivity | Rat, Human, Mouse |
| Clonality | Monoclonal |
| Format | Lyophilized |
| Description | Anti-Actin/ACTA1 Antibody Picoband™ (monoclonal, 3H5) . Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat. |
| Reconstitution | Add 0.2ml of distilled water will yield a concentration of 500ug/ml. |
| Gene ID | 58 |
|---|---|
| Other Names | Actin, alpha skeletal muscle, 3.6.4.-, Alpha-actin-1, Actin, alpha skeletal muscle, intermediate form, ACTA1, ACTA |
| Calculated MW | 43 kDa |
| Application Details | Western blot, 0.25-0.5 µg/ml, Human, Mouse, Rat Immunohistochemistry (Paraffin-embedded Section), 2-5 µg/ml, Human |
| Contents | Each vial contains 4mg Trehalose, 0.9mg NaCl and 0.2mg Na2HPO4. |
| Clone Names | Clone: 3H5 |
| Immunogen | A synthetic peptide corresponding to a sequence at the C-terminus of human Actin, identical to the related mouse and rat sequences. |
| Purification | Immunogen affinity purified. |
| Storage | Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles. |
| Name | ACTA1 |
|---|---|
| Synonyms | ACTA |
| Function | Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. |
| Cellular Location | Cytoplasm, cytoskeleton. |
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Provided below are standard protocols that you may find useful for product applications.
Background
Actin, a highly conserved protein, is a major component of both the cytoskeletal and contractile structures in the cell types. It varies in amount, being related to the type of differentiation and to the functional state of cells and tissues. The actins exhibit over 90% sequence homology, but each isoform has a unique NH2-terminal sequence. The isoforms are comprised of three alpha-actin, one beta-actin, two gamma-actin. Because the amino acid sequence of the C-terminal is the same for almost all actins, this antibody has been raised using a synthetic peptide corresponding to the C-terminal 11 residues.
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