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Anti-liver Arginase/ARG1 Antibody Picoband™ (monoclonal, 2B12)
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| WB, FC |
|---|---|
| Primary Accession | P05089 |
| Host | Mouse |
| Isotype | Mouse IgG2b |
| Reactivity | Rat, Human, Mouse, Monkey |
| Clonality | Monoclonal |
| Format | Lyophilized |
| Description | Anti-liver Arginase/ARG1 Antibody Picoband™ (monoclonal, 2B12) . Tested in Flow Cytometry, WB applications. This antibody reacts with Human, Monkey, Mouse, Rat. |
| Reconstitution | Add 0.2ml of distilled water will yield a concentration of 500 µg/ml. |
| Gene ID | 383 |
|---|---|
| Other Names | Arginase-1, 3.5.3.1, Liver-type arginase, Type I arginase, ARG1 |
| Calculated MW | 35 kDa |
| Application Details | Western blot, 0.1-0.5 µg/ml, Monkey, Mouse, Rat Flow Cytometry, 1-3 µ/1x10^6 cells, Human |
| Contents | Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.01mg NaN3. |
| Clone Names | Clone: 2B12 |
| Immunogen | E.coli-derived human liver Arginase/ARG1 recombinant protein (Position: E25-D183). |
| Purification | Immunogen affinity purified. |
| Storage | Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles. |
| Name | ARG1 |
|---|---|
| Function | Key element of the urea cycle converting L-arginine to urea and L-ornithine, which is further metabolized into metabolites proline and polyamides that drive collagen synthesis and bioenergetic pathways critical for cell proliferation, respectively; the urea cycle takes place primarily in the liver and, to a lesser extent, in the kidneys. |
| Cellular Location | Cytoplasm. Cytoplasmic granule. Note=Localized in azurophil granules of neutrophils (PubMed:15546957) |
| Tissue Location | Within the immune system initially reported to be selectively expressed in granulocytes (polymorphonuclear leukocytes [PMNs]) (PubMed:15546957). Also detected in macrophages mycobacterial granulomas (PubMed:23749634). Expressed in group2 innate lymphoid cells (ILC2s) during lung disease (PubMed:27043409) |
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abcepta to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
Background
ARG1 (arginase, live) is a cytosolic enzyme and expressed predominantly in the liver as a component of the urea cycle. The isoform encoded byARG1, referred to as the liver, or A-I, isoform, contributes 98% of the arginase activity in liver but is also present in red cells. Using a rat liverARG1cDNA clone to probe a human liver cDNA library,Haraguchi et al. (1987)isolated and characterized a cDNA corresponding to theARG1gene.The ARG1 gene is mapped on 6q23.2 and the arginase gene contains 8 exons. By immunologic studies, 90% of the arginase in red blood cell and liver was precipitated by the antibody, whereas only 50% of the arginase in kidney, brain, and the gastrointestinal tract reacted with it. Inherited deficiency of this enzyme results in argininemia, an autosomal recessive disorder characterized by hyperammonemia. Two transcript variants encoding different isoforms have been found for this gene.
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