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Anti-GAD65/GAD2 Antibody Picoband™ (monoclonal, 4E12)
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| WB |
|---|---|
| Primary Accession | Q05329 |
| Host | Mouse |
| Isotype | Mouse IgG1 |
| Reactivity | Rat, Mouse |
| Clonality | Monoclonal |
| Format | Lyophilized |
| Description | Anti-GAD65/GAD2 Antibody Picoband™ (monoclonal, 4E12) . Tested in WB applications. This antibody reacts with Mouse, Rat. |
| Reconstitution | Add 0.2ml of distilled water will yield a concentration of 500ug/ml. |
| Gene ID | 2572 |
|---|---|
| Other Names | Glutamate decarboxylase 2, 4.1.1.15, 65 kDa glutamic acid decarboxylase, GAD-65, Glutamate decarboxylase 65 kDa isoform, GAD2 (HGNC:4093), GAD65 |
| Calculated MW | 65 kDa |
| Application Details | Western blot, 0.1-0.5 µg/ml, Mouse, Rat |
| Contents | Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.01mg NaN3. |
| Clone Names | Clone: 4E12 |
| Immunogen | A synthetic peptide corresponding to a sequence at the N-terminus of human GAD65, different from the related mouse and rat sequences by one amino acid. |
| Purification | Immunogen affinity purified. |
| Storage | Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles. |
| Name | GAD2 (HGNC:4093) |
|---|---|
| Synonyms | GAD65 |
| Function | Catalyzes the production of GABA. |
| Cellular Location | Cytoplasm, cytosol. Cytoplasmic vesicle. Presynaptic cell membrane; Lipid-anchor. Golgi apparatus membrane; Peripheral membrane protein; Cytoplasmic side. Note=Associated to cytoplasmic vesicles In neurons, cytosolic leaflet of Golgi membranes and presynaptic clusters |
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abcepta to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
Background
Glutamate decarboxylase 2, also known as GAD65, is an enzyme that in humans is encoded by the GAD2 gene. This gene encodes one of several forms of glutamic acid decarboxylase, identified as a major autoantigen in insulin-dependent diabetes. The enzyme encoded is responsible for catalyzing the production of gamma-aminobutyric acid from L-glutamic acid. A pathogenic role for this enzyme has been identified in the human pancreas since it has been identified as an autoantibody and an autoreactive T cell target in insulin-dependent diabetes. This gene may also play a role in the stiff man syndrome. Alternative splicing results in multiple transcript variants that encode the same protein.
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