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Anti-HMG4 Antibody Picoband™ (monoclonal, 8H9)
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| WB, IF, ICC, FC |
|---|---|
| Primary Accession | O15347 |
| Host | Mouse |
| Isotype | Mouse IgG2b |
| Reactivity | Rat, Human, Mouse |
| Clonality | Monoclonal |
| Format | Lyophilized |
| Description | Anti-HMG4 Antibody Picoband™ (monoclonal, 8H9) . Tested in Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. |
| Reconstitution | Add 0.2ml of distilled water will yield a concentration of 500 µg/ml. |
| Gene ID | 3149 |
|---|---|
| Other Names | High mobility group protein B3, High mobility group protein 2a, HMG-2a, High mobility group protein 4, HMG-4, HMGB3, HMG2A, HMG4 |
| Calculated MW | 23 kDa |
| Application Details | Western blot, 0.1-0.5 µg/ml, Human, Mouse, Rat Immunocytochemistry/Immunofluorescence, 2 µg/ml, Human Flow Cytometry, 1-3 µg/1x10^6 cells, Human |
| Subcellular Localization | Nucleus. Chromosome. Cytoplasm. |
| Tissue Specificity | Expressed predominantly in placenta. |
| Protein Name | High mobility group protein B3 |
| Contents | Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3. |
| Clone Names | Clone: 8H9 |
| Immunogen | A synthetic peptide corresponding to a sequence at the N-terminus of human HMG4, identical to the related mouse and rat sequences. |
| Purification | Immunogen affinity purified. |
| Cross Reactivity | No cross-reactivity with other proteins. |
| Storage | Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles. |
| Name | HMGB3 |
|---|---|
| Synonyms | HMG2A, HMG4 |
| Function | Multifunctional protein with various roles in different cellular compartments. May act in a redox sensitive manner. Associates with chromatin and binds DNA with a preference for non-canonical DNA structures such as single-stranded DNA. Can bend DNA and enhance DNA flexibility by looping thus providing a mechanism to promote activities on various gene promoters (By similarity). Proposed to be involved in the innate immune response to nucleic acids by acting as a cytoplasmic promiscuous immunogenic DNA/RNA sensor (By similarity). Negatively regulates B-cell and myeloid cell differentiation. In hematopoietic stem cells may regulate the balance between self-renewal and differentiation. Involved in negative regulation of canonical Wnt signaling (By similarity). |
| Cellular Location | Nucleus {ECO:0000250|UniProtKB:P40618, ECO:0000255|PROSITE-ProRule:PRU00267}. Chromosome Cytoplasm {ECO:0000250|UniProtKB:O54879} |
| Tissue Location | Expressed predominantly in placenta. |
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Provided below are standard protocols that you may find useful for product applications.
Background
High-mobility group protein B, also known as HMG4, is a protein that in humans is encoded by the HMGB3 gene. This gene encodes a member of a family of proteins containing one or more high mobility group DNA-binding motifs. The encoded protein plays an important role in maintaining stem cell populations, and may be aberrantly expressed in tumor cells. A mutation in this gene was associated with microphthalmia, syndromic 13. There are numerous pseudogenes of this gene on multiple chromosomes. Alternative splicing results in multiple transcript variants.
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