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Anti-ATP5H in Antibody Picoband™ (monoclonal, 6B12)
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| WB, IHC, IF, ICC |
|---|---|
| Primary Accession | O75947 |
| Host | Mouse |
| Isotype | Mouse IgG2b |
| Reactivity | Rat, Human, Mouse, Monkey |
| Clonality | Monoclonal |
| Format | Lyophilized |
| Description | Anti-ATP5H in Antibody Picoband™ (monoclonal, 6B12) . Tested in IF, IHC, ICC, WB applications. This antibody reacts with Human, Monkey, Mouse, Rat. |
| Reconstitution | Add 0.2ml of distilled water will yield a concentration of 500 µg/ml. |
| Gene ID | 10476 |
|---|---|
| Other Names | ATP synthase subunit d, mitochondrial, ATPase subunit d, ATP synthase peripheral stalk subunit d, ATP5PD (HGNC:845), ATP5H |
| Calculated MW | 22 kDa |
| Application Details | Western blot, 0.1-0.5 µg/ml, Human, Mouse, Rat, Monkey Immunohistochemistry (Paraffin-embedded Section), 0.5-1 µg/ml, Human Immunocytochemistry/Immunofluorescence, 2 µg/ml, Human |
| Subcellular Localization | Mitochondrion. Mitochondrion inner membrane. |
| Protein Name | ATP synthase subunit d, mitochondrial |
| Contents | Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3. |
| Clone Names | Clone: 6B12 |
| Immunogen | E.coli-derived human ATP5H recombinant protein (Position: A2-L161). Human ATP5H shares 81% and 78% amino acid (aa) sequence identity with mouse and rat ATP5H, respectively. |
| Purification | Immunogen affinity purified. |
| Cross Reactivity | No cross-reactivity with other proteins. |
| Storage | Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles. |
| Name | ATP5PD (HGNC:845) |
|---|---|
| Synonyms | ATP5H |
| Function | Mitochondrial membrane ATP synthase (F(1)F(0) ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F(1) - containing the extramembraneous catalytic core, and F(0) - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Part of the complex F(0) domain and the peripheric stalk, which acts as a stator to hold the catalytic alpha(3)beta(3) subcomplex and subunit a/ATP6 static relative to the rotary elements. |
| Cellular Location | Mitochondrion. Mitochondrion inner membrane. |
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Provided below are standard protocols that you may find useful for product applications.
Background
ATP5H is also known as ATPQ. Mitochondrial ATP synthase catalyzes ATP synthesis, utilizing an electrochemical gradient of protons across the inner membrane during oxidative phosphorylation. It is composed of two linked multi-subunit complexes: the soluble catalytic core, F1, and the membrane-spanning component, Fo, which comprises the proton channel. The F1 complex consists of 5 different subunits (alpha, beta, gamma, delta, and epsilon) assembled in a ratio of 3 alpha, 3 beta, and a single representative of the other 3. The Fo seems to have nine subunits (a, b, c, d, e, f, g, F6 and 8). This gene encodes the d subunit of the Fo complex. Alternatively spliced transcript variants encoding different isoforms have been identified for this gene. In addition, three pseudogenes are located on chromosomes 9, 12 and 15.
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