Anti-IRS1 Antibody Picoband™ (monoclonal, 10I3)
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application
| WB, IHC, IF, ICC, FC |
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Primary Accession | P35568 |
Host | Mouse |
Isotype | Mouse IgG2a |
Reactivity | Human |
Clonality | Monoclonal |
Format | Lyophilized |
Description | Anti-IRS1 Antibody Picoband™ (monoclonal, 10I3) . Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human. |
Reconstitution | Add 0.2ml of distilled water will yield a concentration of 500 µg/ml. |
Gene ID | 3667 |
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Other Names | Insulin receptor substrate 1, IRS-1, IRS1 |
Calculated MW | 160-180 kDa |
Application Details | Western blot, 0.1-0.5 µg/ml Immunohistochemistry (Paraffin-embedded Section), 0.5-1 µg/ml Immunocytochemistry/Immunofluorescence, 2 µg/ml Flow Cytometry, 1-3 µg/1x10^6 cells |
Subcellular Localization | Cytosol. Nucleus. Caveola. Insulin receptor complex. Plasma membrane. |
Contents | Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3. |
Clone Names | Clone: 10I3 |
Immunogen | E.coli-derived human IRS1 recombinant protein (Position: S1041-Q1242). Human IRS1 shares 78% and 80% amino acid (aa) sequence identity with mouse and rat IRS1, respectively. |
Cross Reactivity | No cross-reactivity with other proteins. |
Storage | Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles. |
Name | IRS1 |
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Function | May mediate the control of various cellular processes by insulin. When phosphorylated by the insulin receptor binds specifically to various cellular proteins containing SH2 domains such as phosphatidylinositol 3-kinase p85 subunit or GRB2. Activates phosphatidylinositol 3-kinase when bound to the regulatory p85 subunit (By similarity). |
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Provided below are standard protocols that you may find useful for product applications.
Background
Insulin receptor substrate 1 (IRS-1) is a signalling adapter protein that in humans is encoded by the IRS-1 gene. It is mapped to 2q36.3. This gene exhibited no intrinsic enzyme activity, and it can serve as a docking protein involved in binding and activating other signal transduction molecules after being phosphorylated on tyrosine by insulin receptor kinase. IRS1 plays a key role in transmitting signals from the insulin and insulin-like growth factor-1 (IGF-1) receptors to intracellular pathways PI3K/Akt and Erk MAP kinase pathways. IRS1 also has important biological function for both metabolic and mitogenic (growth promoting) pathways. In addition to those, IRS1 is a key regulator of PI3K within malignant cells.
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