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> home > Products > Primary Antibodies > Signal Transduction > Anti-DHFR Antibody Picoband™ (monoclonal, 3C8)
Anti-DHFR Antibody Picoband™ (monoclonal, 3C8)
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Application 
| WB, IHC |
|---|---|
| Primary Accession | P00374 |
| Host | Mouse |
| Isotype | Mouse IgG2b |
| Reactivity | Rat, Human |
| Clonality | Monoclonal |
| Format | Lyophilized |
| Description | Anti-DHFR Antibody Picoband™ (monoclonal, 3C8) . Tested in IHC, WB applications. This antibody reacts with Human, Rat. |
| Reconstitution | Add 0.2ml of distilled water will yield a concentration of 500 µg/ml. |
| Gene ID | 1719 |
|---|---|
| Other Names | Dihydrofolate reductase, 1.5.1.3, DHFR |
| Calculated MW | 22 kDa |
| Application Details | Western blot, 0.1-0.5 µg/ml Immunohistochemistry (Paraffin-embedded Section), 0.5-1 µg/ml |
| Subcellular Localization | Mitochondrion. Cytoplasm |
| Tissue Specificity | Widely expressed in fetal and adult tissues, including throughout the fetal and adult brains and whole blood. Expression is higher in the adult brain than in the fetal brain. |
| Contents | Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3. |
| Clone Names | Clone: 3C8 |
| Immunogen | E.coli-derived human DHFR recombinant protein (Position: V2-D187). Human DHFRshares90%aminoacid (aa)sequenceidentitywith both mouseandrat DHFR. |
| Cross Reactivity | No cross-reactivity with other proteins. |
| Storage | Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles. |
| Name | DHFR |
|---|---|
| Function | Key enzyme in folate metabolism. Contributes to the de novo mitochondrial thymidylate biosynthesis pathway. Catalyzes an essential reaction for de novo glycine and purine synthesis, and for DNA precursor synthesis. Binds its own mRNA and that of DHFR2. |
| Cellular Location | Mitochondrion {ECO:0000250|UniProtKB:P00375}. Cytoplasm {ECO:0000250|UniProtKB:P00375} |
| Tissue Location | Widely expressed in fetal and adult tissues, including throughout the fetal and adult brains and whole blood Expression is higher in the adult brain than in the fetal brain |
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Application Protocols
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$ 370.00
Cat# ABO14841
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