Anti-Human AMPK beta 2 DyLight® 550 conjugated PRKAB2 Antibody(monoclonal, 6G1)
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application
| FC |
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Primary Accession | O43741 |
Host | Mouse |
Isotype | Mouse IgG2b |
Reactivity | Human |
Clonality | Monoclonal |
Format | Liquid |
Description | Anti-Human AMPK beta 2 DyLight® 550 conjugated PRKAB2 Antibody (monoclonal, 6G1) . Tested in Flow Cytometry applications. This antibody reacts with Human. |
Gene ID | 5565 |
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Other Names | 5'-AMP-activated protein kinase subunit beta-2, AMPK subunit beta-2, PRKAB2 |
Calculated MW | 30302 Da |
Application Details | Flow Cytometry, 1-3 µg/1x10^6 cells |
Contents | Each vial contains 50% glycerol, 0.9% NaCl, 0.2% Na2HPO4, 0.02% NaN3. |
Clone Names | Clone: 6G1 |
Immunogen | A synthetic peptide corresponding to a sequence at the N-terminus of human AMPK beta 2, different from the related mouse sequence by three amino acids, and from the related rat sequence by two amino acids. |
Cross Reactivity | No cross-reactivity with other proteins. |
Storage | At -20°C for one year from date of receipt. Avoid repeated freezing and thawing. Protect from light. |
Name | PRKAB2 |
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Function | Non-catalytic subunit of AMP-activated protein kinase (AMPK), an energy sensor protein kinase that plays a key role in regulating cellular energy metabolism. In response to reduction of intracellular ATP levels, AMPK activates energy-producing pathways and inhibits energy-consuming processes: inhibits protein, carbohydrate and lipid biosynthesis, as well as cell growth and proliferation. AMPK acts via direct phosphorylation of metabolic enzymes, and by longer-term effects via phosphorylation of transcription regulators. Also acts as a regulator of cellular polarity by remodeling the actin cytoskeleton; probably by indirectly activating myosin. Beta non-catalytic subunit acts as a scaffold on which the AMPK complex assembles, via its C- terminus that bridges alpha (PRKAA1 or PRKAA2) and gamma subunits (PRKAG1, PRKAG2 or PRKAG3). |
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Background
5'-AMP-activated protein kinase subunit beta-2 is an enzyme that in humans is encoded by the PRKAB2 gene. The protein encoded by this gene is a regulatory subunit of the AMP-activated protein kinase (AMPK). AMPK is a heterotrimer consisting of an alpha catalytic subunit, and non-catalytic beta and gamma subunits. It is an important energy-sensing enzyme that monitors cellular energy status. In response to cellular metabolic stresses, AMPK is activated, and thus phosphorylates and inactivates acetyl-CoA carboxylase (ACC) and beta-hydroxy beta-methylglutaryl-CoA reductase (HMGCR), key enzymes involved in regulating de novo biosynthesis of fatty acid and cholesterol. This subunit may be a positive regulator of AMPK activity. It is highly expressed in skeletal muscle and thus may have tissue-specific roles. Multiple alternatively spliced transcript variants have been found for this gene.
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