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Anti-ACTN3 Antibody Picoband™ (monoclonal, 9B5)
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| WB, IHC |
|---|---|
| Primary Accession | Q08043 |
| Host | Mouse |
| Isotype | Mouse IgG1 |
| Reactivity | Rat, Mouse |
| Clonality | Monoclonal |
| Format | Lyophilized |
| Description | Anti-ACTN3 Antibody Picoband™ (monoclonal, 9B5) . Tested in IHC, WB applications. This antibody reacts with Mouse, Rat. |
| Gene ID | 89 |
|---|---|
| Other Names | Alpha-actinin-3, Alpha-actinin skeletal muscle isoform 3, F-actin cross-linking protein, ACTN3 |
| Calculated MW | 103 kDa |
| Application Details | Western blot, 0.1-0.5 µg/ml Immunohistochemistry (Paraffin-embedded Section), 0.5-1 µg/ml |
| Tissue Specificity | Expressed only in a subset of type 2 skeletal muscle fibers. |
| Contents | Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3. |
| Clone Names | Clone: 9B5 |
| Immunogen | A synthetic peptide corresponding to a sequence at the C-terminus of human ACTN3, different from the related mouse sequence by five amino acids. |
| Cross Reactivity | No cross-reactivity with other proteins. |
| Storage | Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles. |
| Name | ACTN3 |
|---|---|
| Function | F-actin cross-linking protein which is thought to anchor actin to a variety of intracellular structures. This is a bundling protein. |
| Tissue Location | Expression restricted to fast (type 2) skeletal muscle fibers (at protein level). |
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Provided below are standard protocols that you may find useful for product applications.
Background
Alpha-actinin-3, also known as alpha-actinin skeletal muscle isoform 3 or F-actin cross-linking protein, is a protein that in humans is encoded by the ACTN3 gene. This gene encodes a member of the alpha-actin binding protein gene family. The encoded protein is primarily expressed in skeletal muscle and functions as a structural component of sarcomeric Z line. This protein is involved in crosslinking actin containing thin filaments. An allelic polymorphism in this gene results in both coding and non-coding variants; the reference genome represents the coding allele. The non-functional allele of this gene is associated with elite athlete status.
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