Anti-ADA Antibody Picoband™ (monoclonal, 6D4)
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application ![]()
| WB, IHC, FC |
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Primary Accession | P00813 |
Host | Mouse |
Isotype | Mouse IgG2b |
Reactivity | Human |
Clonality | Monoclonal |
Format | Lyophilized |
Description | Anti-ADA Antibody Picoband™ (monoclonal, 6D4) . Tested in Flow Cytometry, IHC, WB applications. This antibody reacts with Human. |
Gene ID | 100 |
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Other Names | Adenosine deaminase, 3.5.4.4, Adenosine aminohydrolase, ADA, ADA1 |
Calculated MW | 45 kDa |
Application Details | Western blot, 0.1-0.5 µg/ml Immunohistochemistry (Paraffin-embedded Section), 0.5-1 µg/ml Flow Cytometry, 1-3 µg/1x10^6 cells |
Subcellular Localization | Cell membrane |
Tissue Specificity | Found in all tissues, occurs in large amounts in T-lymphocytes (PubMed:20959412). Expressed at the time of weaning in gastrointestinal tissues. |
Contents | Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3. |
Clone Names | Clone: 6D4 |
Immunogen | E. coli-derived human ADA recombinant protein (Position: Q135-L363). Human ADA shares 82.5% and 82.9% amino acid (aa) sequence identity with mouse and rat ADA, respectively. |
Cross Reactivity | No cross-reactivity with other proteins. |
Storage | Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles. |
Name | ADA |
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Synonyms | ADA1 |
Function | Catalyzes the hydrolytic deamination of adenosine and 2- deoxyadenosine (PubMed:16670267, PubMed:23193172, PubMed:26166670, PubMed:8452534, PubMed:9361033). Plays an important role in purine metabolism and in adenosine homeostasis. Modulates signaling by extracellular adenosine, and so contributes indirectly to cellular signaling events. Acts as a positive regulator of T-cell coactivation, by binding DPP4 (PubMed:20959412). Its interaction with DPP4 regulates lymphocyte-epithelial cell adhesion (PubMed:11772392). Enhances dendritic cell immunogenicity by affecting dendritic cell costimulatory molecule expression and cytokines and chemokines secretion (By similarity). Enhances CD4+ T-cell differentiation and proliferation (PubMed:20959412). Acts as a positive modulator of adenosine receptors ADORA1 and ADORA2A, by enhancing their ligand affinity via conformational change (PubMed:23193172). Stimulates plasminogen activation (PubMed:15016824). Plays a role in male fertility (PubMed:21919946, PubMed:26166670). Plays a protective role in early postimplantation embryonic development (By similarity). Also responsible for the deamination of cordycepin (3'-deoxyadenosine), a fungal natural product that shows antitumor, antibacterial, antifungal, antivirus, and immune regulation properties (PubMed:26038697). |
Cellular Location | Cell membrane; Peripheral membrane protein; Extracellular side. Cell junction. Cytoplasmic vesicle lumen {ECO:0000250|UniProtKB:P03958}. Cytoplasm. Lysosome. Note=Colocalized with DPP4 at the cell surface. |
Tissue Location | Found in all tissues, occurs in large amounts in T- lymphocytes (PubMed:20959412). Expressed at the time of weaning in gastrointestinal tissues. |

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