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Anti-Thymidylate Synthase TYMS Monoclonal Antibody

     
  •  - Anti-Thymidylate Synthase TYMS Monoclonal Antibody ABO14498
    Figure 1. Western blot analysis of TYMS using anti-TYMS antibody (M04320-1).
    Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
    Lane 1: human Hela whole cell lysates,
    Lane 2: human Jurkat whole cell lysates,
    Lane 3: human 293T whole cell lysates,
    Lane 4: monkey COS-7 whole cell lysates,
    Lane 5: rat thymus tissue lysates,
    Lane 6: rat PC-12 whole cell lysates,
    Lane 7: mouse thymus tissue lysates,
    Lane 8: mouse SP2/0 whole cell lysates.
    After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TYMS antigen affinity purified monoclonal antibody (Catalog # M04320-1) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TYMS at approximately 36 kDa. The expected band size for TYMS is at 36 kDa.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, IHC, IF, ICC, IP, FC
Primary Accession P04818
Host Rabbit
Isotype Rabbit IgG
Reactivity Rat, Human, Mouse, Monkey
Clonality Monoclonal
Format Liquid
Description Anti-Thymidylate Synthase TYMS Monoclonal Antibody . Tested in WB, IHC, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human, Monkey, Mouse, Rat.
Additional Information
Gene ID 7298
Other Names Thymidylate synthase, TS, TSase, 2.1.1.45, TYMS (HGNC:12441), TS
Calculated MW 35716 Da
Application Details WB 1:500-1:2000
IHC 1:50-1:200
ICC/IF 1:50-1:200
IP 1:50
FC 1:50
Contents Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clone Names Clone: ADAA-20
Immunogen A synthesized peptide derived from human Thymidylate Synthase Contributes to the de novo mitochondrial thymidylate biosynthesis pathway.
Purification Affinity-chromatography
Storage Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Protein Information
Name TYMS (HGNC:12441)
Synonyms TS
Function Catalyzes the reductive methylation of 2'-deoxyuridine 5'- monophosphate (dUMP) to thymidine 5'-monophosphate (dTMP), using the cosubstrate, 5,10- methylenetetrahydrofolate (CH2H4folate) as a 1- carbon donor and reductant and contributes to the de novo mitochondrial thymidylate biosynthesis pathway.
Cellular Location Nucleus. Cytoplasm. Mitochondrion. Mitochondrion matrix. Mitochondrion inner membrane
Research Areas
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$ 370.00
Cat# ABO14498
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