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Anti-Methyl-Histone H3 (di K4) HIST1H3A Rabbit Monoclonal Antibody

     
  •  - Anti-Methyl-Histone H3 (di K4) HIST1H3A Rabbit Monoclonal Antibody ABO14306
    Immunohistochemical analysis of paraffin-embedded human liver, using Methyl-Histone H3 (di K4) Antibody(M12477-1)
    HIST1H3A was detected in paraffin-embedded tissue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-HIST1H3A Antibody (M12477-1)overnight at 4℃. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37℃. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
    detail
  •  - Anti-Methyl-Histone H3 (di K4) HIST1H3A Rabbit Monoclonal Antibody ABO14306
    Figure 1. Western blot analysis of Methyl-Histone H3 (di K4) using anti-Methyl-Histone H3 (di K4) antibody (M12477-1).
    Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
    Lane 1: human Hela whole cell lysates,
    Lane 2: human 293T whole cell lysates,
    Lane 3: human CACO-2 whole cell lysates,
    Lane 4: human HepG2 whole cell lysates,
    Lane 5: rat PC-12 whole cell lysates,
    Lane 6: rat C6 whole cell lysates,
    Lane 7: mouse RAW264.7 whole cell lysates,
    Lane 8: mouse NIH/3T3 whole cell lysates.
    After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Methyl-Histone H3 (di K4) antigen affinity purified monoclonal antibody (Catalog # M12477-1) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Methyl-Histone H3 (di K4) at approximately 15 kDa. The expected band size for Methyl-Histone H3 (di K4) is at 15 kDa.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, IHC, IF, ICC, IP, FC
Primary Accession P68431
Host Rabbit
Isotype Rabbit IgG
Reactivity Rat, Human, Mouse
Clonality Monoclonal
Format Liquid
Description Anti-Methyl-Histone H3 (di K4) HIST1H3A Rabbit Monoclonal Antibody . Tested in WB, IHC, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.
Additional Information
Gene ID 8350;8351;8352;8353;8354;8355;8356;8357;8358;8968
Other Names Histone H3.1, Histone H3/a, Histone H3/b, Histone H3/c, Histone H3/d, Histone H3/f, Histone H3/h, Histone H3/i, Histone H3/j, Histone H3/k, Histone H3/l, H3C1 (HGNC:4766), H3FA, HIST1H3A
Calculated MW 15404 MW KDa
Application Details WB 1:500-1:2000
IHC 1:50-1:200
ICC/IF 1:50-1:200
IP 1:50
FC 1:50
Subcellular Localization Nucleus. Chromosome.
Contents Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clone Names Clone: AFF-8
Immunogen A synthesized peptide derived from human Methyl-Histone H3 (di K4)
Purification Affinity-chromatography
Storage Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Protein Information
Name H3C1 (HGNC:4766)
Synonyms H3FA, HIST1H3A
Function Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
Cellular Location Nucleus. Chromosome.
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$ 370.00
Cat# ABO14306
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