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Anti-alpha smooth muscle Actin ACTA2 Rabbit Monoclonal Antibody

     
  •  - Anti-alpha smooth muscle Actin ACTA2 Rabbit Monoclonal Antibody ABO14304
    Immunohistochemical analysis of paraffin-embedded human colon, using alpha smooth muscle Actin Antibody.
    detail
  •  - Anti-alpha smooth muscle Actin ACTA2 Rabbit Monoclonal Antibody ABO14304
    Immunofluorescent analysis of A431 cells, using alpha smooth muscle Actin Antibody .
    detail
  •  - Anti-alpha smooth muscle Actin ACTA2 Rabbit Monoclonal Antibody ABO14304
    Figure 1. Western blot analysis of ACTA2 using anti-ACTA2 antibody (M01072-1).
    Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
    Lane 1: human A549 whole cell lysates,
    Lane 2: human Hela whole cell lysates,
    Lane 3: human 293T whole cell lysates,
    Lane 4: human HepG2 whole cell lysates,
    Lane 5: human Caco-2 whole cell lysates,
    Lane 6: human SH-SY5Y whole cell lysates,
    Lane 7: human U251 whole cell lysates,
    Lane 8: human MCF-7 whole cell lysates.
    After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ACTA2 antigen affinity purified monoclonal antibody (Catalog # M01072-1) at 1:1000 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ACTA2 at approximately 42 kDa. The expected band size for ACTA2 is at 42 kDa.
    detail
  •  - Anti-alpha smooth muscle Actin ACTA2 Rabbit Monoclonal Antibody ABO14304
    Figure 2. Western blot analysis of ACTA2 using anti-ACTA2 antibody (M01072-1).
    Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
    Lane 1: rat heart tissue lysates,
    Lane 2: rat lung tissue lysates,
    Lane 3: rat brain tissue lysates,
    Lane 4: mouse heart tissue lysates,
    Lane 5: mouse lung tissue lysates,
    Lane 6: mouse brain tissue lysates.
    After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ACTA2 antigen affinity purified monoclonal antibody (Catalog # M01072-1) at 1:1000 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ACTA2 at approximately 42 kDa. The expected band size for ACTA2 is at 42 kDa.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, IHC, IF, ICC, FC
Primary Accession P62736
Host Rabbit
Isotype Rabbit IgG
Reactivity Rat, Human, Mouse
Clonality Monoclonal
Format Liquid
Description Anti-alpha smooth muscle Actin ACTA2 Rabbit Monoclonal Antibody . Tested in WB, IHC, ICC/IF, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.
Additional Information
Gene ID 59
Other Names Actin, aortic smooth muscle, 3.6.4.-, Alpha-actin-2, Cell growth-inhibiting gene 46 protein, Actin, aortic smooth muscle, intermediate form, ACTA2, ACTSA, ACTVS
Calculated MW 42009 MW KDa
Application Details WB 1:1000-1:10000
IHC 1:500-1:1000
ICC/IF 1:100-1:500
FC 1:20
Subcellular Localization Cytoplasm, cytoskeleton.
Contents Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clone Names Clone: EO-1
Immunogen A synthesized peptide derived from human alpha smooth muscle Actin
Purification Affinity-chromatography
Storage Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Protein Information
Name ACTA2
Synonyms ACTSA, ACTVS
Function Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.
Cellular Location Cytoplasm, cytoskeleton.
Research Areas
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$ 370.00
Cat# ABO14304
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