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Anti-gamma Sarcoglycan Rabbit Monoclonal Antibody

     
  •  - Anti-gamma Sarcoglycan Rabbit Monoclonal Antibody ABO14248
    Western blot analysis of gamma Sarcoglycan expression in Human skeletal muscle lysate.
    detail
  •  - Anti-gamma Sarcoglycan Rabbit Monoclonal Antibody ABO14248
    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue using anti-gamma Sarcoglycan antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody ( 1/100) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
    detail
  •  - Anti-gamma Sarcoglycan Rabbit Monoclonal Antibody ABO14248
    All lanes use the Antibody at 1:2K dilution for 1 hour at room temperature.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, IHC, IP
Primary Accession Q13326
Host Rabbit
Isotype Rabbit IgG
Reactivity Human
Clonality Monoclonal
Format Liquid
Description Anti-gamma Sarcoglycan Rabbit Monoclonal Antibody . Tested in WB, IHC, IP applications. This antibody reacts with Human.
Additional Information
Gene ID 6445
Other Names Gamma-sarcoglycan, Gamma-SG, 35 kDa dystrophin-associated glycoprotein, 35DAG, SGCG
Calculated MW 32379 MW KDa
Application Details WB 1:500-1:2000
IHC 1:50-1:200
IP 1:50
Subcellular Localization Cell membrane, sarcolemma ; Single-pass type II membrane protein. Cytoplasm, cytoskeleton.
Tissue Specificity Expressed in skeletal and heart muscle.
Contents Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clone Names Clone: AbS34
Immunogen A synthesized peptide derived from human gamma Sarcoglycan
Purification Affinity-chromatography
Storage Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Protein Information
Name SGCG
Function Component of the sarcoglycan complex, a subcomplex of the dystrophin-glycoprotein complex which forms a link between the F-actin cytoskeleton and the extracellular matrix.
Cellular Location Cell membrane, sarcolemma; Single- pass type II membrane protein. Cytoplasm, cytoskeleton
Tissue Location Expressed in skeletal and heart muscle.
Research Areas
Citations (0)
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$ 370.00
Cat# ABO14248
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