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Anti-ATP citrate lyase ACLY Rabbit Monoclonal Antibody

     
  •  - Anti-ATP citrate lyase ACLY Rabbit Monoclonal Antibody ABO14239
    Immunohistochemical analysis of paraffin-embedded human brain carcinoma, using ATP citrate lyase Antibody .
    detail
  •  - Anti-ATP citrate lyase ACLY Rabbit Monoclonal Antibody ABO14239
    Immunoprecipitate (IP) analysis using the Antibody at 1:50 dilution. (wb at 1:3K dilution)
    detail
  •  - Anti-ATP citrate lyase ACLY Rabbit Monoclonal Antibody ABO14239
    Figure 1. Western blot analysis of ACLY using anti-ACLY antibody (M02372).
    Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
    Lane 1: human Hela whole cell lysates,
    Lane 2: human A549 whole cell lysates,
    Lane 3: human HepG2 whole cell lysates,
    Lane 4: rat brain tissue lysates,
    Lane 5: mouse brain tissue lysates,
    Lane 6: mouse kidney tissue lysates.
    After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ACLY antigen affinity purified monoclonal antibody (Catalog # M02372) at 1:1000 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ACLY at approximately 121 kDa. The expected band size for ACLY is at 121 kDa.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, IHC, IF, ICC, IP, FC
Primary Accession P53396
Host Rabbit
Isotype Rabbit IgG
Reactivity Rat, Human, Mouse
Clonality Monoclonal
Format Liquid
Description Anti-ATP citrate lyase ACLY Rabbit Monoclonal Antibody . Tested in WB, IHC, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.
Additional Information
Gene ID 47
Other Names ATP-citrate synthase, 2.3.3.8, ATP-citrate (pro-S-)-lyase, ACL, Citrate cleavage enzyme, ACLY
Calculated MW 120839 MW KDa
Application Details WB 1:1000-1:10000
IHC 1:50-1:200
ICC/IF 1:50-1:200
IP 1:50
FC 1:50
Subcellular Localization Cytoplasm.
Contents Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clone Names Clone: ECF-1
Immunogen A synthesized peptide derived from human ATP citrate lyase
Purification Affinity-chromatography
Storage Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Protein Information
Name ACLY
Function Catalyzes the cleavage of citrate into oxaloacetate and acetyl-CoA, the latter serving as common substrate for de novo cholesterol and fatty acid synthesis.
Cellular Location Cytoplasm, cytosol.
Research Areas
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$ 370.00
Cat# ABO14239
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