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Anti-Apg10 (Atg10) Rabbit Monoclonal Antibody

     
  •  - Anti-Apg10 (Atg10) Rabbit Monoclonal Antibody ABO14169
    Immunoprecipitate (IP) analysis using the Antibody at 1:50 dilution. (wb at 1:3K dilution)
    detail
  •  - Anti-Apg10 (Atg10) Rabbit Monoclonal Antibody ABO14169
    Figure 1. Western blot analysis of Apg10 using anti-Apg10 antibody (M07803).
    Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
    Lane 1: human Hela whole cell lysates,
    Lane 2: human HepG2 whole cell lysates,
    Lane 3: human MCF-7 whole cell lysates,
    Lane 4: human SH-SY5Y whole cell lysates,
    Lane 5: rat brain tissue lysates,
    Lane 6: mouse brain tissue lysates.
    After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Apg10 antigen affinity purified monoclonal antibody (Catalog # M07803) at 1:1000 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Apg10 at approximately 28 kDa. The expected band size for Apg10 is at 25 kDa.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, IP
Primary Accession Q9H0Y0
Host Rabbit
Isotype Rabbit IgG
Reactivity Rat, Human, Mouse
Clonality Monoclonal
Format Liquid
Description Anti-Apg10 (Atg10) Rabbit Monoclonal Antibody . Tested in WB, IP applications. This antibody reacts with Human, Mouse, Rat.
Additional Information
Gene ID 83734
Other Names Ubiquitin-like-conjugating enzyme ATG10, 2.3.2.-, Autophagy-related protein 10, APG10-like, ATG10, APG10L
Calculated MW 25279 MW KDa
Application Details WB 1:500-1:2000
IP 1:50
Subcellular Localization Cytoplasm.
Contents Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clone Names Clone: ABBD-1
Immunogen A synthesized peptide derived from human Apg10 (Atg10)
Purification Affinity-chromatography
Storage Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Protein Information
Name ATG10
Synonyms APG10L
Function E2-like enzyme involved in autophagy. Acts as an E2-like enzyme that catalyzes the conjugation of ATG12 to ATG5. ATG12 conjugation to ATG5 is required for autophagy. Likely serves as an ATG5-recognition molecule. Not involved in ATG12 conjugation to ATG3 (By similarity). Plays a role in adenovirus-mediated cell lysis.
Cellular Location Cytoplasm.
Research Areas
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$ 370.00
Cat# ABO14169
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