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Anti-Synaptophysin SYP Rabbit Monoclonal Antibody

     
  •  - Anti-Synaptophysin SYP Rabbit Monoclonal Antibody ABO14139
    Western blot analysis of Synaptophysin expression in SH-SY5Y cell lysate.
    detail
  •  - Anti-Synaptophysin SYP Rabbit Monoclonal Antibody ABO14139
    Immunohistochemical analysis of paraffin-embedded mouse brain, using Synaptophysin Antibody.
    detail
  •  - Anti-Synaptophysin SYP Rabbit Monoclonal Antibody ABO14139
    Immunofluorescent analysis of PC-12 cells, using Synaptophysin Antibody.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, IHC, IF, ICC
Primary Accession P08247
Host Rabbit
Isotype Rabbit IgG
Reactivity Rat, Human, Mouse
Clonality Monoclonal
Format Liquid
Description Anti-Synaptophysin SYP Rabbit Monoclonal Antibody . Tested in WB, IHC, ICC/IF applications. This antibody reacts with Human, Mouse, Rat.
Additional Information
Gene ID 6855
Other Names Synaptophysin, Major synaptic vesicle protein p38, SYP
Calculated MW 33845 MW KDa
Application Details WB 1:5000-1:10000
IHC 1:50-1:200
ICC/IF 1:50-1:200
Subcellular Localization Cytoplasmic vesicle, secretory vesicle, synaptic vesicle membrane; Multi-pass membrane protein. Cell junction, synapse, synaptosome.
Tissue Specificity Characteristic of a type of small (30-80 nm) neurosecretory vesicles, including presynaptic vesicles, but also vesicles of various neuroendocrine cells of both neuronal and epithelial phenotype.
Contents Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clone Names Clone: BGB-19
Immunogen A synthesized peptide derived from human Synaptophysin
Purification Affinity-chromatography
Storage Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Protein Information
Name SYP
Function Possibly involved in structural functions as organizing other membrane components or in targeting the vesicles to the plasma membrane. Involved in the regulation of short-term and long-term synaptic plasticity (By similarity).
Cellular Location Cytoplasmic vesicle, secretory vesicle, synaptic vesicle membrane; Multi-pass membrane protein. Synapse, synaptosome
Tissue Location Expressed in the brain, with expression in the hippocampus, the neuropil in the dentate gyrus, where expression is higher in the outer half of the molecular layer than in the inner half, and in the neuropil of CA4 and CA3 (PubMed:8838578). Expressed in the putamen (at protein level) (PubMed:17296554)
Research Areas
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$ 370.00
Cat# ABO14139
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