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Anti-Cytochrome C CYCS Rabbit Monoclonal Antibody

     
  •  - Anti-Cytochrome C CYCS Rabbit Monoclonal Antibody ABO14124
    Immunofluorescent analysis using the Antibody at 1:50 dilution.
    detail
  •  - Anti-Cytochrome C CYCS Rabbit Monoclonal Antibody ABO14124
    Immunofluorescent analysis using the Antibody at 1:50 dilution.
    detail
  •  - Anti-Cytochrome C CYCS Rabbit Monoclonal Antibody ABO14124
    Immunofluorescent analysis using the Antibody at 1:50 dilution.
    detail
  •  - Anti-Cytochrome C CYCS Rabbit Monoclonal Antibody ABO14124
    Immunohistochemical analysis of paraffin-embedded human liver, using Cytochrome C Antibody.
    detail
  •  - Anti-Cytochrome C CYCS Rabbit Monoclonal Antibody ABO14124
    Immunofluorescent analysis of Hela cells, using Cytochrome C Antibody.
    detail
  •  - Anti-Cytochrome C CYCS Rabbit Monoclonal Antibody ABO14124
    Figure 1. Western blot analysis of CYCS using anti-CYCS antibody (M03529-2).
    Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
    Lane 1: human Hela whole cell lysates,
    Lane 2: human 293T whole cell lysates,
    Lane 3: human HepG2 whole cell lysates,
    Lane 4: human HT-1080 whole cell lysates,
    Lane 5: rat kidney tissue lysates,
    Lane 6: rat skeletal muscle tissue lysates,
    Lane 7: mouse kidney tissue lysates,
    Lane 8: mouse skeletal muscle tissue lysates.
    After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CYCS antigen affinity purified monoclonal antibody (Catalog # M03529-2) at 1:1000 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CYCS at approximately 14 kDa. The expected band size for CYCS is at 12 kDa.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, IHC, IF, ICC, IP
Primary Accession P99999
Host Rabbit
Isotype Rabbit IgG
Reactivity Rat, Human, Mouse
Clonality Monoclonal
Format Liquid
Description Anti-Cytochrome C CYCS Rabbit Monoclonal Antibody . Tested in WB, IHC, ICC/IF, IP applications. This antibody reacts with Human, Mouse, Rat.
Additional Information
Gene ID 54205
Other Names Cytochrome c, CYCS, CYC
Calculated MW 11749 MW KDa
Application Details WB 1:1000-1:2000
IHC 1:50-1:200
ICC/IF 1:50-1:200
IP 1:30
Subcellular Localization Mitochondrion intermembrane space. Loosely associated with the inner membrane.
Contents Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clone Names Clone: GOC-3
Immunogen A synthesized peptide derived from human Cytochrome C
Purification Affinity-chromatography
Storage Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Protein Information
Name CYCS
Synonyms CYC
Function Electron carrier protein. The oxidized form of the cytochrome c heme group can accept an electron from the heme group of the cytochrome c1 subunit of cytochrome reductase. Cytochrome c then transfers this electron to the cytochrome oxidase complex, the final protein carrier in the mitochondrial electron-transport chain.
Cellular Location Mitochondrion intermembrane space. Note=Loosely associated with the inner membrane
Research Areas
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$ 370.00
Cat# ABO14124
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