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Anti-DAP Kinase 1 DAPK1 Rabbit Monoclonal Antibody

     
  •  - Anti-DAP Kinase 1 DAPK1 Rabbit Monoclonal Antibody ABO14116
    Western blot analysis of DAP Kinase 1 expression in HeLa cell lysate.
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  •  - Anti-DAP Kinase 1 DAPK1 Rabbit Monoclonal Antibody ABO14116
    Immunohistochemical analysis of paraffin-embedded human breast cancer, using DAP Kinase 1 Antibody.
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  •  - Anti-DAP Kinase 1 DAPK1 Rabbit Monoclonal Antibody ABO14116
    Immunofluorescent analysis of Hela cells, using DAP Kinase 1 Antibody.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, IHC, IF, ICC
Primary Accession P53355
Host Rabbit
Isotype Rabbit IgG
Reactivity Rat, Human, Mouse
Clonality Monoclonal
Format Liquid
Description Anti-DAP Kinase 1 DAPK1 Rabbit Monoclonal Antibody . Tested in WB, IHC, ICC/IF applications. This antibody reacts with Human, Mouse, Rat.
Additional Information
Gene ID 1612
Other Names Death-associated protein kinase 1, DAP kinase 1, 2.7.11.1, DAPK1, DAPK
Calculated MW 160046 MW KDa
Application Details WB 1:500-1:2000
IHC 1:50-1:200
ICC/IF 1:50-1:200
Subcellular Localization Isoform 1: Cytoplasm. Cytoplasm, cytoskeleton. Colocalizes with MAP1B in the microtubules and cortical actin fibers.
Tissue Specificity Isoform 2 is expressed in normal intestinal tissue as well as in colorectal carcinomas..
Contents Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clone Names Clone: COA-4
Immunogen A synthesized peptide derived from human DAP Kinase 1
Purification Affinity-chromatography
Storage Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Protein Information
Name DAPK1
Synonyms DAPK
Function Calcium/calmodulin-dependent serine/threonine kinase involved in multiple cellular signaling pathways that trigger cell survival, apoptosis, and autophagy. Regulates both type I apoptotic and type II autophagic cell deaths signal, depending on the cellular setting. The former is caspase-dependent, while the latter is caspase-independent and is characterized by the accumulation of autophagic vesicles. Phosphorylates PIN1 resulting in inhibition of its catalytic activity, nuclear localization, and cellular function. Phosphorylates TPM1, enhancing stress fiber formation in endothelial cells. Phosphorylates STX1A and significantly decreases its binding to STXBP1. Phosphorylates PRKD1 and regulates JNK signaling by binding and activating PRKD1 under oxidative stress. Phosphorylates BECN1, reducing its interaction with BCL2 and BCL2L1 and promoting the induction of autophagy. Phosphorylates TSC2, disrupting the TSC1-TSC2 complex and stimulating mTORC1 activity in a growth factor-dependent pathway. Phosphorylates RPS6, MYL9 and DAPK3. Acts as a signaling amplifier of NMDA receptors at extrasynaptic sites for mediating brain damage in stroke. Cerebral ischemia recruits DAPK1 into the NMDA receptor complex and it phosphorylates GRINB at Ser-1303 inducing injurious Ca(2+) influx through NMDA receptor channels, resulting in an irreversible neuronal death. Required together with DAPK3 for phosphorylation of RPL13A upon interferon-gamma activation which is causing RPL13A involvement in transcript-selective translation inhibition.
Cellular Location [Isoform 1]: Cytoplasm. Cytoplasm, cytoskeleton. Note=Colocalizes with MAP1B in the microtubules and cortical actin fibers
Tissue Location Isoform 2 is expressed in normal intestinal tissue as well as in colorectal carcinomas.
Research Areas
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$ 370.00
Cat# ABO14116
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