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Anti-Flotillin 1 FLOT1 Rabbit Monoclonal Antibody

     
  •  - Anti-Flotillin 1 FLOT1 Rabbit Monoclonal Antibody ABO14068
    Figure 1. Western blot analysis of Flotillin 1 using anti-Flotillin 1 antibody (M05980).
    Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
    Lane 1: human U2OS whole cell lysates,
    Lane 2: human 293T whole cell lysates,
    Lane 3: human A431 whole cell lysates,
    Lane 4: human PC-3 whole cell lysates,
    Lane 5: rat brain tissue lysates,
    Lane 6: rat lung tissue lysates,
    Lane 7: mouse brain tissue lysates,
    Lane 8: mouse lung tissue lysates.
    After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Flotillin 1 antigen affinity purified monoclonal antibody (Catalog # M05980) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Flotillin 1 at approximately 47 kDa. The expected band size for Flotillin 1 is at 47 kDa.
    detail
  •  - Anti-Flotillin 1 FLOT1 Rabbit Monoclonal Antibody ABO14068
    Figure 2. IHC analysis of Flotillin 1 using anti-Flotillin 1 antibody (M05980).
    Flotillin 1 was detected in a paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-Flotillin 1 Antibody (M05980) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
    detail
  •  - Anti-Flotillin 1 FLOT1 Rabbit Monoclonal Antibody ABO14068
    Figure 3. IHC analysis of Flotillin 1 using anti-Flotillin 1 antibody (M05980).
    Flotillin 1 was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-Flotillin 1 Antibody (M05980) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
    detail
  •  - Anti-Flotillin 1 FLOT1 Rabbit Monoclonal Antibody ABO14068
    Figure 4. IHC analysis of Flotillin 1 using anti-Flotillin 1 antibody (M05980).
    Flotillin 1 was detected in a paraffin-embedded section of human lung squamous cell carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-Flotillin 1 Antibody (M05980) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, IHC, IP
Primary Accession O75955
Host Rabbit
Isotype Rabbit IgG
Reactivity Rat, Human, Mouse
Clonality Monoclonal
Format Liquid
Description Anti-Flotillin 1 FLOT1 Rabbit Monoclonal Antibody . Tested in WB, IHC, IP applications. This antibody reacts with Human, Mouse, Rat.
Additional Information
Gene ID 10211
Other Names Flotillin-1, FLOT1
Calculated MW 47355 MW KDa
Application Details WB 1:500-1:2000
IHC 1:50-1:200
IP 1:50
Subcellular Localization Cell membrane; Peripheral membrane protein. Membrane, caveola; Peripheral membrane protein. Melanosome. Endosome. Membrane-associated protein of caveolae. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
Contents Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clone Names Clone: BHB-8
Immunogen A synthesized peptide derived from human Flotillin 1
Purification Affinity-chromatography
Storage Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Protein Information
Name FLOT1
Function May act as a scaffolding protein within caveolar membranes, functionally participating in formation of caveolae or caveolae-like vesicles.
Cellular Location Cell membrane; Peripheral membrane protein. Endosome Membrane, caveola {ECO:0000250|UniProtKB:O08917}; Peripheral membrane protein {ECO:0000250|UniProtKB:O08917}. Melanosome. Membrane raft. Note=Identified by mass spectrometry in melanosome fractions from stage I to stage IV (PubMed:17081065) Membrane-associated protein of caveola (By similarity) {ECO:0000250|UniProtKB:O08917, ECO:0000269|PubMed:17081065}
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$ 370.00
Cat# ABO14068
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