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Anti-E Cadherin Rabbit Monoclonal Antibody

     
  •  - Anti-E Cadherin Rabbit Monoclonal Antibody ABO14014
    All lanes use the Antibody at 1:6W dilution for 1 hour at room temperature.
    detail
  •  - Anti-E Cadherin Rabbit Monoclonal Antibody ABO14014
    Immunofluorescent analysis using the Antibody at 1:50 dilution.
    detail
  •  - Anti-E Cadherin Rabbit Monoclonal Antibody ABO14014
    Western blot analysis of E-cadherin expression in MCF7 cell lysate.
    detail
  •  - Anti-E Cadherin Rabbit Monoclonal Antibody ABO14014
    Immunohistochemical analysis of paraffin-embedded human colon cancer, using E Cadherin Antibody.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, IHC, IF, ICC, FC
Primary Accession P12830
Host Rabbit
Isotype Rabbit IgG
Reactivity Human
Clonality Monoclonal
Format Liquid
Description Anti-E Cadherin Rabbit Monoclonal Antibody . Tested in WB, IHC, ICC/IF, Flow Cytometry applications. This antibody reacts with Human.
Additional Information
Gene ID 999
Other Names Cadherin-1 {ECO:0000312|HGNC:HGNC:1748}, CAM 120/80, Epithelial cadherin, E-cadherin {ECO:0000312|HGNC:HGNC:1748}, Uvomorulin, CD324, E-Cad/CTF1, E-Cad/CTF2, E-Cad/CTF3, CDH1 (HGNC:1748)
Calculated MW 97456 MW KDa
Application Details WB 1:5000-1:10000
IHC 1:100-1:500
ICC/IF 1:100-1:500
FC 1:500
Tissue Specificity Non-neural epithelial tissues.
Contents Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clone Names Clone: EA-20
Immunogen A synthesized peptide derived from human E Cadherin
Purification Affinity-chromatography
Storage Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Protein Information
Name CDH1 (HGNC:1748)
Function Cadherins are calcium-dependent cell adhesion proteins (PubMed:11976333). They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. CDH1 is involved in mechanisms regulating cell-cell adhesions, mobility and proliferation of epithelial cells (PubMed:11976333). Promotes organization of radial actin fiber structure and cellular response to contractile forces, via its interaction with AMOTL2 which facilitates anchoring of radial actin fibers to CDH1 junction complexes at the cell membrane (By similarity). Has a potent invasive suppressor role. It is a ligand for integrin alpha-E/beta-7.
Cellular Location Cell junction, adherens junction. Cell membrane; Single-pass type I membrane protein Endosome. Golgi apparatus, trans-Golgi network. Cytoplasm {ECO:0000250|UniProtKB:P09803}. Cell junction, desmosome. Note=Colocalizes with DLGAP5 at sites of cell-cell contact in intestinal epithelial cells. Anchored to actin microfilaments through association with alpha-, beta- and gamma- catenin. Sequential proteolysis induced by apoptosis or calcium influx, results in translocation from sites of cell-cell contact to the cytoplasm. Colocalizes with RAB11A endosomes during its transport from the Golgi apparatus to the plasma membrane. Recruited to desmosomes at the initial assembly phase and also accumulates progressively at mature desmosome cell-cell junctions (PubMed:25208567)
Tissue Location Expressed in granuloma macrophages (at protein level) (PubMed:27760340). Expressed in the liver (PubMed:3263290)
Research Areas
Citations (0)
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$ 370.00
Cat# ABO14014
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