Anti-HP1 alpha CBX5 Rabbit Monoclonal Antibody
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application ![]()
| WB, IHC, IF, ICC, IP, FC |
---|---|
Primary Accession | P45973 |
Host | Rabbit |
Isotype | Rabbit IgG |
Reactivity | Rat, Human, Mouse |
Clonality | Monoclonal |
Format | Liquid |
Description | Anti-HP1 alpha CBX5 Rabbit Monoclonal Antibody . Tested in WB, IHC, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat. |
Gene ID | 23468 |
---|---|
Other Names | Chromobox protein homolog 5, Antigen p25, Heterochromatin protein 1 homolog alpha, HP1 alpha, CBX5, HP1A |
Calculated MW | 22225 MW KDa |
Application Details | WB 1:500-1:2000 IHC 1:50-1:200 ICC/IF 1:50-1:200 IP 1:50 FC 1:50 |
Subcellular Localization | Nucleus. Chromosome. Chromosome, centromere. Component of centromeric and pericentromeric heterochromatin. Associates with chromosomes during mitosis. Associates specifically with chromatin during metaphase and anaphase. |
Contents | Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA. |
Clone Names | Clone: DHO-3 |
Immunogen | A synthesized peptide derived from human HP1 alpha |
Purification | Affinity-chromatography |
Storage | Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles. |
Name | CBX5 |
---|---|
Synonyms | HP1A |
Function | Component of heterochromatin that recognizes and binds histone H3 tails methylated at 'Lys-9' (H3K9me), leading to epigenetic repression. In contrast, it is excluded from chromatin when 'Tyr-41' of histone H3 is phosphorylated (H3Y41ph). Can interact with lamin-B receptor (LBR). This interaction can contribute to the association of the heterochromatin with the inner nuclear membrane. Involved in the formation of functional kinetochore through interaction with MIS12 complex proteins. |
Cellular Location | Nucleus. Chromosome. Chromosome, centromere Note=Colocalizes with HNRNPU in the nucleus (PubMed:19617346) Component of centromeric and pericentromeric heterochromatin Associates with chromosomes during mitosis. Associates specifically with chromatin during metaphase and anaphase (PubMed:19617346) Localizes to sites of DNA damage (PubMed:28977666) |
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