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Anti-Lysozyme LYZ Rabbit Monoclonal Antibody

     
  •  - Anti-Lysozyme LYZ Rabbit Monoclonal Antibody ABO13924
    Figure 1. Western blot analysis of Lysozyme using anti-Lysozyme antibody (M01811).
    Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
    Lane 1: human HL-60 whole cell lysates,
    Lane 2: human U937 whole cell lysates,
    Lane 3: mouse RAW264.7 whole cell lysates.
    After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Lysozyme antigen affinity purified monoclonal antibody (Catalog # M01811) at 1:5000 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Lysozyme at approximately 15 kDa. The expected band size for Lysozyme is at 17 kDa.
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  •  - Anti-Lysozyme LYZ Rabbit Monoclonal Antibody ABO13924
    Immunohistochemical analysis of paraffin-embedded mouse kidney, using Lysozyme Antibody.
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  •  - Anti-Lysozyme LYZ Rabbit Monoclonal Antibody ABO13924
    Immunohistochemical analysis of paraffin-embedded Rat cerebral cortex, using the Antibody at 1:2000 dilution.
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  •  - Anti-Lysozyme LYZ Rabbit Monoclonal Antibody ABO13924
    Immunohistochemical analysis of paraffin-embedded Human thyroid cancer, using the Antibody at 1:4000 dilution.
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  •  - Anti-Lysozyme LYZ Rabbit Monoclonal Antibody ABO13924
    Immunohistochemical analysis of paraffin-embedded Human placenta, using the Antibody at 1:4000 dilution.
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  •  - Anti-Lysozyme LYZ Rabbit Monoclonal Antibody ABO13924
    Immunohistochemical analysis of paraffin-embedded Mouse skin, using the Antibody at 1:2000 dilution.
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  •  - Anti-Lysozyme LYZ Rabbit Monoclonal Antibody ABO13924
    Immunohistochemical analysis of paraffin-embedded Mouse heart, using the Antibody at 1:2000 dilution.
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  •  - Anti-Lysozyme LYZ Rabbit Monoclonal Antibody ABO13924
    Immunofluorescent analysis using the Antibody at 1:50 dilution.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, IHC, IF, ICC, IP
Primary Accession P61626
Host Rabbit
Isotype Rabbit IgG
Reactivity Rat, Human, Mouse
Clonality Monoclonal
Format Liquid
Description Anti-Lysozyme LYZ Rabbit Monoclonal Antibody . Tested in WB, IHC, ICC/IF, IP applications. This antibody reacts with Human, Mouse, Rat.
Additional Information
Gene ID 4069
Other Names Lysozyme C, 3.2.1.17, 1, 4-beta-N-acetylmuramidase C, LYZ, LZM
Calculated MW 16537 MW KDa
Application Details WB 1:5000-1:10000
IHC 1:50-1:200
ICC/IF 1:50-1:200
IP 1:20
Subcellular Localization Secreted.
Contents Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clone Names Clone: EBO-12
Immunogen A synthesized peptide derived from human Lysozyme
Purification Affinity-chromatography
Storage Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Protein Information
Name LYZ
Synonyms LZM
Function Lysozymes have primarily a bacteriolytic function; those in tissues and body fluids are associated with the monocyte-macrophage system and enhance the activity of immunoagents.
Cellular Location Secreted.
Research Areas
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$ 370.00
Cat# ABO13924
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