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Anti-Vimentin Rabbit Monoclonal Antibody

     
  •  - Anti-Vimentin Rabbit Monoclonal Antibody ABO13913
    Immunohistochemical analysis of paraffin-embedded Human lung large cell cancer, using the Antibody at 1:1000 dilution.
    detail
  •  - Anti-Vimentin Rabbit Monoclonal Antibody ABO13913
    Immunohistochemical analysis of paraffin-embedded Human lung adenocarcinoma, using the Antibody at 1:1000 dilution.
    detail
  •  - Anti-Vimentin Rabbit Monoclonal Antibody ABO13913
    Immunohistochemical analysis of paraffin-embedded human colon, using Vimentin Antibody.
    detail
  •  - Anti-Vimentin Rabbit Monoclonal Antibody ABO13913
    Immunofluorescent analysis of Hela cells, using Vimentin Antibody.
    detail
  •  - Anti-Vimentin Rabbit Monoclonal Antibody ABO13913
    Figure 1. Western blot analysis of Vimentin using anti-Vimentin antibody (M00235-1).
    Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
    Lane 1: human 293T whole cell lysates,
    Lane 2: human U251 whole cell lysates,
    Lane 3: human U-87MG whole cell lysates,
    Lane 4: human Jurkat whole cell lysates,
    Lane 5: rat lung tissue lysates,
    Lane 6: mouse lung tissue lysates.
    After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Vimentin antigen affinity purified monoclonal antibody (Catalog # M00235-1) at 1:1000 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Vimentin at approximately 54 kDa. The expected band size for Vimentin is at 54 kDa.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, IHC, IF, ICC, FC
Primary Accession P08670
Host Rabbit
Isotype Rabbit IgG
Reactivity Rat, Human, Mouse
Clonality Monoclonal
Format Liquid
Description Anti-Vimentin Rabbit Monoclonal Antibody . Tested in WB, IHC, ICC/IF, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.
Additional Information
Gene ID 7431
Other Names Vimentin, VIM
Calculated MW 53652 MW KDa
Application Details WB 1:1000-1:5000
IHC 1:200-1:1000
ICC/IF 1:50-1:200
FC 1:50
Subcellular Localization Cytoplasm.
Tissue Specificity Highly expressed in fibroblasts, some expression in T- and B-lymphocytes, and little or no expression in Burkitt's lymphoma cell lines. Expressed in many hormone- independent mammary carcinoma cell lines..
Contents Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clone Names Clone: AGF-22
Immunogen A synthesized peptide derived from human Vimentin
Purification Affinity-chromatography
Storage Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Protein Information
Name VIM (HGNC:12692)
Function Vimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is attached to the nucleus, endoplasmic reticulum, and mitochondria, either laterally or terminally. Plays a role in cell directional movement, orientation, cell sheet organization and Golgi complex polarization at the cell migration front (By similarity). Protects SCRIB from proteasomal degradation and facilitates its localization to intermediate filaments in a cell contact-mediated manner (By similarity).
Cellular Location Cytoplasm. Cytoplasm, cytoskeleton. Nucleus matrix {ECO:0000250|UniProtKB:P31000}. Cell membrane {ECO:0000250|UniProtKB:P20152}
Tissue Location Highly expressed in fibroblasts, some expression in T- and B-lymphocytes, and little or no expression in Burkitt's lymphoma cell lines. Expressed in many hormone-independent mammary carcinoma cell lines.
Research Areas
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$ 370.00
Cat# ABO13913
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