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Anti-TOMM20/Tom20 Rabbit Monoclonal Antibody

     
  •  - Anti-TOMM20/Tom20 Rabbit Monoclonal Antibody ABO13830
    Immunofluorescent analysis using the Antibody at 1:50 dilution.
    detail
  •  - Anti-TOMM20/Tom20 Rabbit Monoclonal Antibody ABO13830
    Immunofluorescent analysis using the Antibody at 1:50 dilution.
    detail
  •  - Anti-TOMM20/Tom20 Rabbit Monoclonal Antibody ABO13830
    Immunofluorescent analysis using the Antibody at 1:150 dilution.
    detail
  •  - Anti-TOMM20/Tom20 Rabbit Monoclonal Antibody ABO13830
    Immunofluorescent analysis using the Antibody at 1:500 dilution.
    detail
  •  - Anti-TOMM20/Tom20 Rabbit Monoclonal Antibody ABO13830
    Immunofluorescent analysis of Hela cells, using TOMM20 Antibody .
    detail
  •  - Anti-TOMM20/Tom20 Rabbit Monoclonal Antibody ABO13830
    Figure 1. Western blot analysis of TOMM20/Tom20 using anti-TOMM20/Tom20 antibody (M04039).
    Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
    Lane 1: human HepG2 whole cell lysates,
    Lane 2: human 293T whole cell lysates,
    Lane 3: human K562 whole cell lysates,
    Lane 4: human Hela whole cell lysates,
    Lane 5: rat kidney tissue lysates,
    Lane 6: rat stomach tissue lysates,
    Lane 7: mouse kidney tissue lysates,
    Lane 8: mouse stomach tissue lysates.
    After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TOMM20/Tom20 antigen affinity purified monoclonal antibody (Catalog # M04039) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TOMM20/Tom20 at approximately 16 kDa. The expected band size for TOMM20/Tom20 is at 16 kDa.
    detail
  •  - Anti-TOMM20/Tom20 Rabbit Monoclonal Antibody ABO13830
    Figure 2. IHC analysis of TOMM20 using anti-TOMM20 antibody (M04039).
    TOMM20 was detected in a paraffin-embedded section of human colorectal adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-TOMM20 Antibody (M04039) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
    detail
  •  - Anti-TOMM20/Tom20 Rabbit Monoclonal Antibody ABO13830
    Figure 3. IHC analysis of TOMM20 using anti-TOMM20 antibody (M04039).
    TOMM20 was detected in a paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-TOMM20 Antibody (M04039) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, IHC, IF, ICC, FC
Primary Accession Q15388
Host Rabbit
Isotype Rabbit IgG
Reactivity Rat, Human, Mouse
Clonality Monoclonal
Format Liquid
Description Anti-TOMM20/Tom20 Rabbit Monoclonal Antibody . Tested in WB, IHC, ICC/IF, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.
Additional Information
Gene ID 9804
Other Names Mitochondrial import receptor subunit TOM20 homolog, Mitochondrial 20 kDa outer membrane protein, Outer mitochondrial membrane receptor Tom20, TOMM20, KIAA0016
Calculated MW 16298 MW KDa
Application Details WB 1:500-1:2000
IHC 1:50-1:200
ICC/IF 1:50-1:200
FC 1:100
Subcellular Localization Mitochondrion outer membrane ; Single-pass membrane protein.
Contents Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clone Names Clone: EOC-20
Immunogen A synthesized peptide derived from human TOMM20
Purification Affinity-chromatography
Storage Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Protein Information
Name TOMM20
Synonyms KIAA0016
Function Central component of the receptor complex responsible for the recognition and translocation of cytosolically synthesized mitochondrial preproteins. Together with TOM22 functions as the transit peptide receptor at the surface of the mitochondrion outer membrane and facilitates the movement of preproteins into the TOM40 translocation pore (By similarity). Required for the translocation across the mitochondrial outer membrane of cytochrome P450 monooxygenases.
Cellular Location Mitochondrion outer membrane; Single-pass membrane protein
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$ 370.00
Cat# ABO13830
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