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Anti-ACTN2 Rabbit Monoclonal Antibody

     
  •  - Anti-ACTN2 Rabbit Monoclonal Antibody ABO13737
    Western blot analysis of ACTN2 expression in HepG2 cell lysate.
    detail
  •  - Anti-ACTN2 Rabbit Monoclonal Antibody ABO13737
    Figure 1. Western blot analysis of ACTN2 using anti-ACTN2 antibody (M03673).
    Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
    Lane 1: rat skeletal muscle tissue lysates,
    Lane 2: rat heart tissue lysates,
    Lane 3: mouse skeletal muscle tissue lysates,
    Lane 4: mouse heart tissue lysates.
    After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ACTN2 antigen affinity purified monoclonal antibody (Catalog # M03673) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ACTN2 at approximately 104 kDa. The expected band size for ACTN2 is at 104 kDa.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, IHC, IP
Primary Accession P35609
Host Rabbit
Isotype Rabbit IgG
Reactivity Rat, Human, Mouse
Clonality Monoclonal
Format Liquid
Description Anti-ACTN2 Rabbit Monoclonal Antibody . Tested in WB, IHC, IP applications. This antibody reacts with Human, Mouse, Rat.
Additional Information
Gene ID 88
Other Names Alpha-actinin-2, Alpha-actinin skeletal muscle isoform 2, F-actin cross-linking protein, ACTN2
Calculated MW 103854 MW KDa
Application Details WB 1:500-1:2000
IHC 1:50-1:200
IP 1:50
Subcellular Localization Cytoplasm, myofibril, sarcomere, Z line. Colocalizes with MYOZ1 and FLNC at the Z-lines of skeletal muscle.
Tissue Specificity Expressed in both skeletal and cardiac muscle.
Contents Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clone Names Clone: AOCB-1
Immunogen A synthesized peptide derived from human ACTN2
Purification Affinity-chromatography
Storage Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Protein Information
Name ACTN2
Function F-actin cross-linking protein which is thought to anchor actin to a variety of intracellular structures. This is a bundling protein.
Cellular Location Cytoplasm, myofibril, sarcomere, Z line. Note=Colocalizes with MYOZ1 and FLNC at the Z-lines of skeletal muscle
Tissue Location Expressed in both skeletal and cardiac muscle.
Research Areas
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$ 370.00
Cat# ABO13737
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