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Anti-Hsp60 HSPD1 Rabbit Monoclonal Antibody

     
  •  - Anti-Hsp60 HSPD1 Rabbit Monoclonal Antibody ABO13632
    All lanes use the Antibody at 1:1W dilution for 1 hour at room temperature.
    detail
  •  - Anti-Hsp60 HSPD1 Rabbit Monoclonal Antibody ABO13632
    All lanes use the Antibody at 1:1W dilution for 1 hour at room temperature.
    detail
  •  - Anti-Hsp60 HSPD1 Rabbit Monoclonal Antibody ABO13632
    All lanes use the Antibody at 1:1W dilution for 1 hour at room temperature.
    detail
  •  - Anti-Hsp60 HSPD1 Rabbit Monoclonal Antibody ABO13632
    All lanes use the Antibody at 1:1W dilution for 1 hour at room temperature.
    detail
  •  - Anti-Hsp60 HSPD1 Rabbit Monoclonal Antibody ABO13632
    Western blot analysis of Hsp60 expression in (1) HeLa cell lysate; (2) 293T cell lysate; (3) NIH/3T3 cell lysate; (4) Mouse heart lysate; (5) PC-12 cell lysate; (6) Rat heart lysate.
    detail
  •  - Anti-Hsp60 HSPD1 Rabbit Monoclonal Antibody ABO13632
    Immunohistochemical analysis of paraffin-embedded human kidney, using Hsp60 Antibody.
    detail
  • SPECIFICATION
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, IHC
Primary Accession P10809
Host Rabbit
Isotype Rabbit IgG
Reactivity Rat, Human, Mouse
Clonality Monoclonal
Format Liquid
Description Anti-Hsp60 HSPD1 Rabbit Monoclonal Antibody . Tested in WB, IHC applications. This antibody reacts with Human, Mouse, Rat.
Additional Information
Gene ID 3329
Other Names 60 kDa heat shock protein, mitochondrial, 5.6.1.7, 60 kDa chaperonin, Chaperonin 60, CPN60, Heat shock protein 60, HSP-60, Hsp60, Heat shock protein family D member 1, HuCHA60, Mitochondrial matrix protein P1, P60 lymphocyte protein, HSPD1, HSP60
Calculated MW 61055 MW KDa
Application Details WB 1:500-1:2000
IHC 1:50-1:200
Subcellular Localization Mitochondrion matrix.
Contents Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clone Names Clone: DOI-8
Immunogen A synthesized peptide derived from human Hsp60
Purification Affinity-chromatography
Storage Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Protein Information
Name HSPD1
Synonyms HSP60
Function Chaperonin implicated in mitochondrial protein import and macromolecular assembly. Together with Hsp10, facilitates the correct folding of imported proteins. May also prevent misfolding and promote the refolding and proper assembly of unfolded polypeptides generated under stress conditions in the mitochondrial matrix (PubMed:11422376, PubMed:1346131). The functional units of these chaperonins consist of heptameric rings of the large subunit Hsp60, which function as a back- to-back double ring. In a cyclic reaction, Hsp60 ring complexes bind one unfolded substrate protein per ring, followed by the binding of ATP and association with 2 heptameric rings of the co-chaperonin Hsp10. This leads to sequestration of the substrate protein in the inner cavity of Hsp60 where, for a certain period of time, it can fold undisturbed by other cell components. Synchronous hydrolysis of ATP in all Hsp60 subunits results in the dissociation of the chaperonin rings and the release of ADP and the folded substrate protein (Probable).
Cellular Location Mitochondrion matrix.
Research Areas
Citations (0)
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$ 370.00
Cat# ABO13632
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