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Anti-STAT6 Rabbit Monoclonal Antibody

     
  •  - Anti-STAT6 Rabbit Monoclonal Antibody ABO13625
    Immunohistochemical analysis of paraffin-embedded Rat stomach, using the Antibody at 1:100 dilution.
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  •  - Anti-STAT6 Rabbit Monoclonal Antibody ABO13625
    Immunohistochemical analysis of paraffin-embedded Human thyroid cancer, using the Antibody at 1:300 dilution.
    detail
  •  - Anti-STAT6 Rabbit Monoclonal Antibody ABO13625
    Immunohistochemical analysis of paraffin-embedded Mouse lung, using the Antibody at 1:100 dilution.
    detail
  •  - Anti-STAT6 Rabbit Monoclonal Antibody ABO13625
    Immunohistochemical analysis of paraffin-embedded rat liver, using STAT6 Antibody.
    detail
  •  - Anti-STAT6 Rabbit Monoclonal Antibody ABO13625
    Immunofluorescent analysis of Hela cells, using STAT6 Antibody .
    detail
  •  - Anti-STAT6 Rabbit Monoclonal Antibody ABO13625
    Immunoprecipitate (IP) analysis using the Antibody at 1:50 dilution. (wb at 1:1K dilution)
    detail
  •  - Anti-STAT6 Rabbit Monoclonal Antibody ABO13625
    Figure 1. Western blot analysis of STAT6 using anti-STAT6 antibody (M00523).
    Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
    Lane 1: human Raji whole cell lysates,
    Lane 2: human Hela whole cell lysates,
    Lane 3: mouse thymus tissue lysates.
    After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-STAT6 antigen affinity purified monoclonal antibody (Catalog # M00523) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for STAT6 at approximately 100 kDa. The expected band size for STAT6 is at 94 kDa.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, IHC, IF, ICC, IP
Primary Accession P42226
Host Rabbit
Isotype Rabbit IgG
Reactivity Human, Mouse
Clonality Monoclonal
Format Liquid
Description Anti-STAT6 Rabbit Monoclonal Antibody . Tested in WB, IHC, ICC/IF, IP applications. This antibody reacts with Human, Mouse.
Additional Information
Gene ID 6778
Other Names Signal transducer and activator of transcription 6, IL-4 Stat, STAT6
Calculated MW 94135 MW KDa
Application Details WB 1:500-1:2000
IHC 1:50-1:200
ICC/IF 1:50-1:200
IP 1:50
Subcellular Localization Cytoplasm. Nucleus. Translocated into the nucleus in response to phosphorylation.
Contents Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clone Names Clone: CFH-19
Immunogen A synthesized peptide derived from human STAT6
Purification Affinity-chromatography
Storage Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Protein Information
Name STAT6
Function Carries out a dual function: signal transduction and activation of transcription. Involved in IL4/interleukin-4- and IL3/interleukin-3-mediated signaling.
Cellular Location Cytoplasm. Nucleus. Note=Translocated into the nucleus in response to phosphorylation
Research Areas
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$ 370.00
Cat# ABO13625
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