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Anti-Actin ACTA1 Rabbit Monoclonal Antibody

     
  •  - Anti-Actin ACTA1 Rabbit Monoclonal Antibody ABO13566
    Immunofluorescent analysis of Hela cells, using Actin Antibody.
    detail
  •  - Anti-Actin ACTA1 Rabbit Monoclonal Antibody ABO13566
    Immunofluorescent analysis using the Antibody at 1:50 dilution.
    detail
  •  - Anti-Actin ACTA1 Rabbit Monoclonal Antibody ABO13566
    Immunofluorescent analysis using the Antibody at 1:50 dilution.
    detail
  •  - Anti-Actin ACTA1 Rabbit Monoclonal Antibody ABO13566
    Figure 1. Western blot analysis of ACTA1 using anti-ACTA1 antibody (M02014-1).
    Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
    Lane 1: human HepG2 whole cell lysates,
    Lane 2: human Caco-2 whole cell lysates,
    Lane 3: human 293T whole cell lysates,
    Lane 4: human SH-SY5Y whole cell lysates,
    Lane 5: rat heart tissue lysates,
    Lane 6: rat small intestine tissue lysates,
    Lane 7: mouse heart tissue lysates,
    Lane 8: mouse small intestine tissue lysates.
    After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ACTA1 antigen affinity purified monoclonal antibody (Catalog # M02014-1) at 1:3000 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ACTA1 at approximately 42 kDa. The expected band size for ACTA1 is at 42 kDa.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, IHC, IF, ICC, IP, FC
Primary Accession P68133
Host Rabbit
Isotype Rabbit IgG
Reactivity Rat, Human, Mouse
Clonality Monoclonal
Format Liquid
Description Anti-Actin ACTA1 Rabbit Monoclonal Antibody . Tested in WB, IHC, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.
Additional Information
Gene ID 58
Other Names Actin, alpha skeletal muscle, 3.6.4.-, Alpha-actin-1, Actin, alpha skeletal muscle, intermediate form, ACTA1, ACTA
Calculated MW 42051 MW KDa
Application Details WB 1:3000-1:20000
IHC 1:50-1:200
ICC/IF 1:50-1:200
IP 1:50
FC 1:50
Subcellular Localization Cytoplasm, cytoskeleton.
Contents Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clone Names Clone: BE-1
Immunogen A synthesized peptide derived from human Actin
Purification Affinity-chromatography
Storage Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Protein Information
Name ACTA1
Synonyms ACTA
Function Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.
Cellular Location Cytoplasm, cytoskeleton.
Research Areas
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$ 370.00
Cat# ABO13566
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