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Anti-G6PD/Glucose 6 Phosphate Dehydrogenase Rabbit Monoclonal Antibody

     
  •  - Anti-G6PD/Glucose 6 Phosphate Dehydrogenase Rabbit Monoclonal Antibody ABO13552
    Figure 1. Western blot analysis of G6PD using anti-G6PD antibody (M00287).
    Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
    Lane 1: human MCF-7 whole cell lysates,
    Lane 2: human A549 whole cell lysates,
    Lane 3: human Hela whole cell lysates.
    After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-G6PD antigen affinity purified monoclonal antibody (Catalog # M00287) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:1000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for G6PD at approximately 59 kDa. The expected band size for G6PD is at 59 kDa.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, IHC, IF, ICC, FC
Primary Accession P11413
Host Rabbit
Isotype Rabbit IgG
Reactivity Human
Clonality Monoclonal
Format Liquid
Description Anti-G6PD/Glucose 6 Phosphate Dehydrogenase Rabbit Monoclonal Antibody . Tested in WB, IHC, ICC/IF, Flow Cytometry applications. This antibody reacts with Human.
Additional Information
Gene ID 2539
Other Names Glucose-6-phosphate 1-dehydrogenase, G6PD, 1.1.1.49, G6PD
Calculated MW 59257 MW KDa
Application Details WB 1:500-1:2000
IHC 1:50-1:200
ICC/IF 1:50-1:200
FC 1:100
Tissue Specificity Isoform Long is found in lymphoblasts, granulocytes and sperm.
Contents Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clone Names Clone: ABBI-7
Immunogen A synthesized peptide derived from human G6PD
Purification Affinity-chromatography
Storage Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Protein Information
Name G6PD
Function Catalyzes the rate-limiting step of the oxidative pentose- phosphate pathway, which represents a route for the dissimilation of carbohydrates besides glycolysis. The main function of this enzyme is to provide reducing power (NADPH) and pentose phosphates for fatty acid and nucleic acid synthesis.
Cellular Location Cytoplasm, cytosol. Membrane; Peripheral membrane protein
Tissue Location Isoform Long is found in lymphoblasts, granulocytes and sperm
Research Areas
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$ 370.00
Cat# ABO13552
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