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Anti-GPX1/Glutathione Peroxidase 1 Rabbit Monoclonal Antibody

     
  •  - Anti-GPX1/Glutathione Peroxidase 1 Rabbit Monoclonal Antibody ABO13493
    Figure 1. Western blot analysis of GPX1 using anti-GPX1 antibody (M01019-1).
    Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
    Lane 1: human 293T whole cell lysates,
    Lane 2: human THP-1 whole cell lysates,
    Lane 3: human HepG2 whole cell lysates,
    Lane 4: rat liver tissue lysates,
    Lane 5: mouse liver tissue lysates.
    After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GPX1 antigen affinity purified monoclonal antibody (Catalog # M01019-1) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:1000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GPX1 at approximately 22 kDa. The expected band size for GPX1 is at 22 kDa.
    detail
  •  - Anti-GPX1/Glutathione Peroxidase 1 Rabbit Monoclonal Antibody ABO13493
    Figure 2. IHC analysis of GPX1 using anti-GPX1 antibody (M01019-1).
    GPX1 was detected in a paraffin-embedded section of human lung squamous cell carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-GPX1 Antibody (M01019-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
    detail
  •  - Anti-GPX1/Glutathione Peroxidase 1 Rabbit Monoclonal Antibody ABO13493
    Figure 3. IHC analysis of GPX1 using anti-GPX1 antibody (M01019-1).
    GPX1 was detected in a paraffin-embedded section of human spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-GPX1 Antibody (M01019-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
    detail
  •  - Anti-GPX1/Glutathione Peroxidase 1 Rabbit Monoclonal Antibody ABO13493
    Figure 4. IHC analysis of GPX1 using anti-GPX1 antibody (M01019-1).
    GPX1 was detected in a paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-GPX1 Antibody (M01019-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, IHC, IP
Primary Accession P07203
Host Rabbit
Isotype Rabbit IgG
Reactivity Rat, Human, Mouse
Clonality Monoclonal
Format Liquid
Description Anti-GPX1/Glutathione Peroxidase 1 Rabbit Monoclonal Antibody . Tested in WB, IHC, IP applications. This antibody reacts with Human, Mouse, Rat.
Additional Information
Gene ID 2876
Other Names Glutathione peroxidase 1, GPx-1, GSHPx-1, 1.11.1.9, Cellular glutathione peroxidase, Phospholipid-hydroperoxide glutathione peroxidase GPX1, 1.11.1.12, GPX1 (HGNC:4553)
Calculated MW 22088 MW KDa
Application Details WB 1:500-1:2000
IHC 1:50-1:200
IP 1:50
Subcellular Localization Cytoplasm.
Contents Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clone Names Clone: IHO-7
Immunogen A synthesized peptide derived from human GPX1
Purification Affinity-chromatography
Storage Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Protein Information
Name GPX1 (HGNC:4553)
Function Catalyzes the reduction of hydroperoxides in a glutathione- dependent manner thus regulating cellular redox homeostasis (PubMed:11115402, PubMed:36608588). Can reduce small soluble hydroperoxides such as H2O2, cumene hydroperoxide and tert-butyl hydroperoxide, as well as several fatty acid-derived hydroperoxides (PubMed:11115402, PubMed:36608588). In platelets catalyzes the reduction of 12-hydroperoxyeicosatetraenoic acid, the primary product of the arachidonate 12-lipoxygenase pathway (PubMed:11115402).
Cellular Location Cytoplasm {ECO:0000250|UniProtKB:P11352}. Mitochondrion {ECO:0000250|UniProtKB:P11352}
Tissue Location Expressed in platelets (at protein level).
Research Areas
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$ 370.00
Cat# ABO13493
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