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> home > Products > Primary Antibodies > Cancer > Anti-TOP1/Topoisomerase I Rabbit Monoclonal Antibody
Anti-TOP1/Topoisomerase I Rabbit Monoclonal Antibody
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Application 
| WB, IHC, IF, ICC, FC |
|---|---|
| Primary Accession | P11387 |
| Host | Rabbit |
| Isotype | Rabbit IgG |
| Reactivity | Human, Mouse |
| Clonality | Monoclonal |
| Format | Liquid |
| Description | Anti-TOP1/Topoisomerase I Rabbit Monoclonal Antibody . Tested in WB, IHC, ICC/IF, Flow Cytometry applications. This antibody reacts with Human, Mouse. |
| Gene ID | 7150 |
|---|---|
| Other Names | DNA topoisomerase 1, 5.6.2.1 {ECO:0000255|PROSITE-ProRule:PRU10130, ECO:0000269|PubMed:14594810, ECO:0000269|PubMed:16033260, ECO:0000269|PubMed:2833744}, DNA topoisomerase I, TOP1 |
| Calculated MW | 90726 MW KDa |
| Application Details | WB 1:1000-1:2000 IHC 1:50-1:200 ICC/IF 1:50-1:200 FC 1:50 |
| Subcellular Localization | Nucleus, nucleolus. Nucleus, nucleoplasm. Diffuse nuclear localization with some enrichment in nucleoli. On CPT treatment, cleared from nucleoli into nucleoplasm. Sumolyated forms found in both nucleoplasm and nucleoli. |
| Tissue Specificity | Endothelial cells.. |
| Contents | Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA. |
| Clone Names | Clone: FIG-20 |
| Immunogen | A synthesized peptide derived from human TOP1 |
| Purification | Affinity-chromatography |
| Storage | Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles. |
| Name | TOP1 |
|---|---|
| Function | Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(3'-phosphotyrosyl)- enzyme intermediate and the expulsion of a 5'-OH DNA strand. The free DNA strand then rotates around the intact phosphodiester bond on the opposing strand, thus removing DNA supercoils. Finally, in the religation step, the DNA 5'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone (By similarity). Regulates the alternative splicing of tissue factor (F3) pre-mRNA in endothelial cells. Involved in the circadian transcription of the core circadian clock component BMAL1 by altering the chromatin structure around the ROR response elements (ROREs) on the BMAL1 promoter. |
| Cellular Location | Nucleus, nucleolus. Nucleus, nucleoplasm. Note=Diffuse nuclear localization with some enrichment in nucleoli. On CPT treatment, cleared from nucleoli into nucleoplasm. Sumoylated forms found in both nucleoplasm and nucleoli |
| Tissue Location | Endothelial cells.. |
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Application Protocols
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$ 370.00
Cat# ABO13425
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