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Anti-NeuN RBFOX3 Rabbit Monoclonal Antibody

     
  •  - Anti-NeuN RBFOX3 Rabbit Monoclonal Antibody ABO13380
    Western blot analysis of NeuN expression in human fetal brain lysate.
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  •  - Anti-NeuN RBFOX3 Rabbit Monoclonal Antibody ABO13380
    Western blot analysis of NeuN using anti-NeuN antibody (M11954).
    Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
    Lane 1: rat brain tissue lysates,
    Lane 2: mouse brain tissue lysates.
    After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-NeuN antigen affinity purified monoclonal antibody (Catalog # M11954) at 1:1000 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NeuN at approximately 46-55 kDa. The expected band size for NeuN is at 34 kDa.
    detail
  •  - Anti-NeuN RBFOX3 Rabbit Monoclonal Antibody ABO13380
    Immunohistochemical analysis of paraffin-embedded Rat testis, using the Antibody at 1:1000 dilution.
    detail
  •  - Anti-NeuN RBFOX3 Rabbit Monoclonal Antibody ABO13380
    Immunohistochemical analysis of paraffin-embedded Human Hodgkin's lymphoma, using the Antibody at 1:500 dilution.
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  •  - Anti-NeuN RBFOX3 Rabbit Monoclonal Antibody ABO13380
    Immunohistochemical analysis of paraffin-embedded Human astrocytoma, using the Antibody at 1:500 dilution.
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  •  - Anti-NeuN RBFOX3 Rabbit Monoclonal Antibody ABO13380
    Immunohistochemical analysis of paraffin-embedded Human stomach, using the Antibody at 1:500 dilution.
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  •  - Anti-NeuN RBFOX3 Rabbit Monoclonal Antibody ABO13380
    Immunohistochemical analysis of paraffin-embedded Mouse testis, using the Antibody at 1:1000 dilution.
    detail
  •  - Anti-NeuN RBFOX3 Rabbit Monoclonal Antibody ABO13380
    Immunofluorescent analysis using the Antibody at 1:150 dilution.
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  •  - Anti-NeuN RBFOX3 Rabbit Monoclonal Antibody ABO13380
    Immunofluorescent analysis using the Antibody at 1:50 dilution.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, IHC, IF, ICC, FC
Primary Accession A6NFN3
Host Rabbit
Isotype Rabbit IgG
Reactivity Rat, Human, Mouse
Clonality Monoclonal
Format Liquid
Description Anti-NeuN RBFOX3 Rabbit Monoclonal Antibody . Tested in WB, IHC, ICC/IF, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.
Additional Information
Gene ID 146713
Other Names RNA binding protein fox-1 homolog 3, Fox-1 homolog C, Neuronal nuclei antigen, NeuN antigen, RBFOX3
Calculated MW 33873 MW KDa
Application Details WB 1:1000-1:2000
IHC 1:500-1:2000
ICC/IF 1:50-1:200
FC 1:50
Subcellular Localization Nucleus. Cytoplasm.
Contents Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clone Names Clone: AO-18
Immunogen A synthesized peptide derived from human NeuN
Purification Affinity-chromatography
Storage Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Protein Information
Name RBFOX3
Function Pre-mRNA alternative splicing regulator. Regulates alternative splicing of RBFOX2 to enhance the production of mRNA species that are targeted for nonsense-mediated decay (NMD).
Cellular Location Nucleus. Cytoplasm. Note=Largely restricted to neuronal nuclei. However, significant cytoplasmic localization in neurons from brains from HIV-infected individuals with cognitive impairment
Research Areas
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$ 370.00
Cat# ABO13380
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