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Anti-CCR7 Rabbit Monoclonal Antibody

     
  •  - Anti-CCR7 Rabbit Monoclonal Antibody ABO13357
    Immunohistochemical analysis of paraffin-embedded human spleen, using CCR7 Antibody.
    detail
  •  - Anti-CCR7 Rabbit Monoclonal Antibody ABO13357
    Immunofluorescent analysis of Hela cells, using CCR7 Antibody .
    detail
  •  - Anti-CCR7 Rabbit Monoclonal Antibody ABO13357
    Figure 1. Western blot analysis of CCR7 using anti-CCR7 antibody (M00390).
    Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
    Lane 1: human MCF-7 whole cell lysates,
    Lane 2: human K562 whole cell lysates,
    Lane 3: rat spleen tissue lysates,
    Lane 4: rat lung tissue lysates,
    Lane 5: rat C6 whole cell lysates,
    Lane 6: mouse spleen tissue lysates,
    Lane 7: mouse lung tissue lysates.
    After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CCR7 antigen affinity purified monoclonal antibody (Catalog # M00390) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CCR7 at approximately 43 kDa. The expected band size for CCR7 is at 43 kDa.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, IHC, IF, ICC, IP
Primary Accession P32248
Host Rabbit
Isotype Rabbit IgG
Reactivity Rat, Human, Mouse
Clonality Monoclonal
Format Liquid
Description Anti-CCR7 Rabbit Monoclonal Antibody . Tested in WB, IHC, ICC/IF, IP applications. This antibody reacts with Human, Mouse, Rat.
Additional Information
Gene ID 1236
Other Names C-C chemokine receptor type 7, C-C CKR-7, CC-CKR-7, CCR-7, BLR2, CDw197, Epstein-Barr virus-induced G-protein coupled receptor 1, EBI1, EBV-induced G-protein coupled receptor 1, MIP-3 beta receptor, CD197, CCR7, CMKBR7, EBI1, EVI1
Calculated MW 42874 MW KDa
Application Details WB 1:500-1:2000
IHC 1:50-1:200
ICC/IF 1:50-1:200
IP 1:50
Subcellular Localization Cell membrane; Multi-pass membrane protein.
Tissue Specificity Expressed in various lymphoid tissues and activated B- and T-lymphocytes, strongly up-regulated in B-cells infected with Epstein-Barr virus and T-cells infected with herpesvirus 6 or 7.
Contents Rabbit IgG in phosphate buffered saline, pH 7.4, 150 mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5 mg/ml BSA.
Clone Names Clone: CFO-3
Immunogen A synthesized peptide derived from human CCR7
Purification Affinity-chromatography
Storage Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Protein Information
Name CCR7
Synonyms CMKBR7, EBI1, EVI1
Function Receptor for the MIP-3-beta chemokine. Probable mediator of EBV effects on B-lymphocytes or of normal lymphocyte functions.
Cellular Location Cell membrane; Multi-pass membrane protein.
Tissue Location Expressed in various lymphoid tissues and activated B- and T-lymphocytes, strongly up-regulated in B-cells infected with Epstein-Barr virus and T-cells infected with herpesvirus 6 or 7
Research Areas
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$ 370.00
Cat# ABO13357
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