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Anti-MCL1 Rabbit Monoclonal Antibody

     
  •  - Anti-MCL1 Rabbit Monoclonal Antibody ABO13328
    Immunohistochemical analysis of paraffin-embedded Human thyroid cancer, using the Antibody at 1:100 dilution.
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  •  - Anti-MCL1 Rabbit Monoclonal Antibody ABO13328
    Immunohistochemical analysis of paraffin-embedded Human glioblastoma, using the Antibody at 1:100 dilution.
    detail
  •  - Anti-MCL1 Rabbit Monoclonal Antibody ABO13328
    Immunofluorescent analysis using the Antibody at 1:150 dilution.
    detail
  •  - Anti-MCL1 Rabbit Monoclonal Antibody ABO13328
    Immunohistochemical analysis of paraffin-embedded human stomach, using MCL1 Antibody.
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  •  - Anti-MCL1 Rabbit Monoclonal Antibody ABO13328
    Figure 1. Western blot analysis of MCL1 using anti-MCL1 antibody (M00712).
    Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
    Lane 1: human Raji whole cell lysates,
    Lane 2: human MCF-7 whole cell lysates,
    Lane 3: human 293T whole cell lysates,
    Lane 4: rat PC-12 whole cell lysates,
    Lane 5: rat C6 whole cell lysates.
    After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MCL1 antigen affinity purified monoclonal antibody (Catalog # M00712) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MCL1 at approximately 37 kDa. The expected band size for MCL1 is at 37 kDa.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, IHC, IF, ICC, IP, FC
Primary Accession Q07820
Host Rabbit
Isotype Rabbit IgG
Reactivity Rat, Human, Mouse
Clonality Monoclonal
Format Liquid
Description Anti-MCL1 Rabbit Monoclonal Antibody . Tested in WB, IHC, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.
Additional Information
Gene ID 4170
Other Names Induced myeloid leukemia cell differentiation protein Mcl-1, Bcl-2-like protein 3, Bcl2-L-3, Bcl-2-related protein EAT/mcl1, mcl1/EAT, MCL1, BCL2L3
Calculated MW 37337 MW KDa
Application Details WB 1:500-1:2000
IHC 1:50-1:200
ICC/IF 1:50-1:200
IP 1:50
FC 1:50
Subcellular Localization Membrane ; Single-pass membrane protein. Cytoplasm. Mitochondrion. Nucleus, nucleoplasm. Cytoplasmic, associated with mitochondria.
Contents Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clone Names Clone: BCO-13
Immunogen A synthesized peptide derived from human MCL1
Purification Affinity-chromatography
Storage Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Protein Information
Name MCL1
Synonyms BCL2L3
Function Involved in the regulation of apoptosis versus cell survival, and in the maintenance of viability but not of proliferation. Mediates its effects by interactions with a number of other regulators of apoptosis. Isoform 1 inhibits apoptosis. Isoform 2 promotes apoptosis.
Cellular Location Membrane; Single-pass membrane protein. Cytoplasm. Mitochondrion. Nucleus, nucleoplasm Note=Cytoplasmic, associated with mitochondria
Research Areas
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$ 370.00
Cat# ABO13328
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