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Anti-ENO1/Alpha Enolase Rabbit Monoclonal Antibody

     
  •  - Anti-ENO1/Alpha Enolase Rabbit Monoclonal Antibody ABO13306
    Western blot analysis of ENO1 in (1)MCF-7 whole cell lysate; (2)Rat brain tissue lysate.
    detail
  •  - Anti-ENO1/Alpha Enolase Rabbit Monoclonal Antibody ABO13306
    Immunohistochemical analysis of paraffin-embedded human colon, using ENO1 Antibody.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, IF, ICC, IP
Primary Accession P06733
Host Rabbit
Isotype Rabbit IgG
Reactivity Rat, Human, Mouse
Clonality Monoclonal
Format Liquid
Description Anti-ENO1/Alpha Enolase Rabbit Monoclonal Antibody . Tested in WB, ICC/IF, IP applications. This antibody reacts with Human, Mouse, Rat.
Additional Information
Gene ID 2023
Other Names Alpha-enolase, 4.2.1.11, 2-phospho-D-glycerate hydro-lyase, C-myc promoter-binding protein, Enolase 1, MBP-1, MPB-1, Non-neural enolase, NNE, Phosphopyruvate hydratase, Plasminogen-binding protein, ENO1, ENO1L1, MBPB1, MPB1
Calculated MW 47169 MW KDa
Application Details WB 1:500-1:2000
ICC/IF 1:50-1:200
IP 1:50
Subcellular Localization Cytoplasm. Cell membrane. Cytoplasm, myofibril, sarcomere, M line. Can translocate to the plasma membrane in either the homodimeric (alpha/alpha) or heterodimeric (alpha/gamma) form. ENO1 is localized to the M line.
Tissue Specificity The alpha/alpha homodimer is expressed in embryo and in most adult tissues. The alpha/beta heterodimer and the beta/beta homodimer are found in striated muscle, and the alpha/gamma heterodimer and the gamma/gamma homodimer in neurons.
Contents Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clone Names Clone: AEA-5
Immunogen A synthesized peptide derived from human ENO1
Purification Affinity-chromatography
Storage Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Protein Information
Name ENO1
Synonyms ENO1L1, MBPB1, MPB1
Function Glycolytic enzyme the catalyzes the conversion of 2- phosphoglycerate to phosphoenolpyruvate (PubMed:1369209, PubMed:29775581). In addition to glycolysis, involved in various processes such as growth control, hypoxia tolerance and allergic responses (PubMed:10802057, PubMed:12666133, PubMed:2005901, PubMed:29775581). May also function in the intravascular and pericellular fibrinolytic system due to its ability to serve as a receptor and activator of plasminogen on the cell surface of several cell-types such as leukocytes and neurons (PubMed:12666133). Stimulates immunoglobulin production (PubMed:1369209).
Cellular Location Cytoplasm. Cell membrane. Cytoplasm, myofibril, sarcomere, M line. Note=Can translocate to the plasma membrane in either the homodimeric (alpha/alpha) or heterodimeric (alpha/gamma) form. ENO1 is localized to the M line
Tissue Location The alpha/alpha homodimer is expressed in embryo and in most adult tissues. The alpha/beta heterodimer and the beta/beta homodimer are found in striated muscle, and the alpha/gamma heterodimer and the gamma/gamma homodimer in neurons
Research Areas
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$ 370.00
Cat# ABO13306
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