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Anti-CCR9 Rabbit Monoclonal Antibody

     
  •  - Anti-CCR9 Rabbit Monoclonal Antibody ABO13297
    Immunofluorescent analysis using the Antibody at 1:50 dilution.
    detail
  •  - Anti-CCR9 Rabbit Monoclonal Antibody ABO13297
    Figure 1. Western blot analysis of CCR9 using anti-CCR9 antibody (M02348).
    Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
    Lane 1: human MOLT-4 whole cell lysates,
    Lane 2: human Jurkat whole cell lysates,
    Lane 3: human U-87MG whole cell lysates,
    Lane 4: human K562 whole cell lysates,
    Lane 5: rat thymus tissue lysates,
    Lane 6: rat PC-12 whole cell lysates,
    Lane 7: mouse thymus tissue lysates.
    After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CCR9 antigen affinity purified monoclonal antibody (Catalog # M02348) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CCR9 at approximately 42 kDa. The expected band size for CCR9 is at 42 kDa.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, IF, ICC, FC
Primary Accession P51686
Host Rabbit
Isotype Rabbit IgG
Reactivity Rat, Human, Mouse
Clonality Monoclonal
Format Liquid
Description Anti-CCR9 Rabbit Monoclonal Antibody . Tested in WB, ICC/IF, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.
Additional Information
Gene ID 10803
Other Names C-C chemokine receptor type 9, C-C CKR-9, CC-CKR-9, CCR-9, G-protein coupled receptor 28, GPR-9-6, CDw199, CCR9, GPR28
Calculated MW 42016 MW KDa
Application Details WB 1:500-1:2000
ICC/IF 1:50-1:200
FC 1:50
Subcellular Localization Cell membrane; Multi-pass membrane protein.
Tissue Specificity Highly expressed in the thymus and low in lymph nodes and spleen..
Contents Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clone Names Clone: AAO-3
Immunogen A synthesized peptide derived from human CCR9
Purification Affinity-chromatography
Storage Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Protein Information
Name CCR9
Synonyms GPR28
Function Receptor for chemokine SCYA25/TECK. Subsequently transduces a signal by increasing the intracellular calcium ions level.
Cellular Location Cell membrane; Multi-pass membrane protein
Tissue Location Highly expressed in the thymus and low in lymph nodes and spleen.
Research Areas
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$ 370.00
Cat# ABO13297
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