Anti-MIF Rabbit Monoclonal Antibody
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application
| WB, IP, FC |
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Primary Accession | P14174 |
Host | Rabbit |
Isotype | Rabbit IgG |
Reactivity | Rat, Human, Mouse |
Clonality | Monoclonal |
Format | Liquid |
Description | Anti-MIF Rabbit Monoclonal Antibody . Tested in WB, IP, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat. |
Gene ID | 4282 |
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Other Names | Macrophage migration inhibitory factor, MIF, 5.3.2.1, Glycosylation-inhibiting factor, L-dopachrome isomerase, L-dopachrome tautomerase, 5.3.3.12, Phenylpyruvate tautomerase, MIF {ECO:0000303|PubMed:2552447, ECO:0000312|HGNC:HGNC:7097} |
Calculated MW | 12476 MW KDa |
Application Details | WB 1:500-1:2000 IP 1:50 FC 1:50 |
Subcellular Localization | Secreted. Cytoplasm. Does not have a cleavable signal sequence and is secreted via a specialized, non- classical pathway. Secreted by macrophages upon stimulation by bacterial lipopolysaccharide (LPS), or by M.tuberculosis antigens. |
Contents | Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA. |
Clone Names | Clone: ABAG-13 |
Immunogen | A synthesized peptide derived from human MIF |
Purification | Affinity-chromatography |
Storage | Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles. |
Name | MIF {ECO:0000303|PubMed:2552447, ECO:0000312|HGNC:HGNC:7097} |
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Function | Pro-inflammatory cytokine involved in the innate immune response to bacterial pathogens (PubMed:15908412, PubMed:17443469, PubMed:23776208). The expression of MIF at sites of inflammation suggests a role as mediator in regulating the function of macrophages in host defense (PubMed:15908412, PubMed:17443469, PubMed:23776208). Counteracts the anti-inflammatory activity of glucocorticoids (PubMed:15908412, PubMed:17443469, PubMed:23776208). Has phenylpyruvate tautomerase and dopachrome tautomerase activity (in vitro), but the physiological substrate is not known (PubMed:11439086, PubMed:17526494). It is not clear whether the tautomerase activity has any physiological relevance, and whether it is important for cytokine activity (PubMed:11439086, PubMed:17526494). |
Cellular Location | Secreted. Cytoplasm. Note=Does not have a cleavable signal sequence and is secreted via a specialized, non-classical pathway Secreted by macrophages upon stimulation by bacterial lipopolysaccharide (LPS), or by M.tuberculosis antigens |
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