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Anti-IDE/Insulysin Rabbit Monoclonal Antibody

     
  •  - Anti-IDE/Insulysin Rabbit Monoclonal Antibody ABO13269
    Western blot analysis of IDE expression in HepG2 cell lysate.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, IHC
Primary Accession P14735
Host Rabbit
Isotype Rabbit IgG
Reactivity Rat, Human, Mouse
Clonality Monoclonal
Format Liquid
Description Anti-IDE/Insulysin Rabbit Monoclonal Antibody . Tested in WB, IHC applications. This antibody reacts with Human, Mouse, Rat.
Additional Information
Gene ID 3416
Other Names Insulin-degrading enzyme, 3.4.24.56, Abeta-degrading protease, Insulin protease, Insulinase, Insulysin, IDE {ECO:0000303|PubMed:20364150, ECO:0000312|HGNC:HGNC:5381}
Calculated MW 117968 MW KDa
Application Details WB 1:500-1:2000
IHC 1:50-1:100
Subcellular Localization Cytoplasm. Cell membrane. Secreted. Present at the cell surface of neuron cells. The membrane-associated isoform is approximately 5 kDa larger than the known cytosolic isoform.
Contents Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clone Names Clone: AOOB-9
Immunogen A synthesized peptide derived from human IDE
Purification Affinity-chromatography
Storage Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Protein Information
Name IDE {ECO:0000303|PubMed:20364150, ECO:0000312|HGNC:HGNC:5381}
Function Plays a role in the cellular breakdown of insulin, APP peptides, IAPP peptides, natriuretic peptides, glucagon, bradykinin, kallidin, and other peptides, and thereby plays a role in intercellular peptide signaling (PubMed:10684867, PubMed:17051221, PubMed:17613531, PubMed:18986166, PubMed:19321446, PubMed:21098034, PubMed:2293021, PubMed:23922390, PubMed:24847884, PubMed:26394692, PubMed:26968463, PubMed:29596046). Substrate binding induces important conformation changes, making it possible to bind and degrade larger substrates, such as insulin (PubMed:23922390, PubMed:26394692, PubMed:29596046). Contributes to the regulation of peptide hormone signaling cascades and regulation of blood glucose homeostasis via its role in the degradation of insulin, glucagon and IAPP (By similarity). Plays a role in the degradation and clearance of APP-derived amyloidogenic peptides that are secreted by neurons and microglia (Probable) (PubMed:26394692, PubMed:9830016). Degrades the natriuretic peptides ANP, BNP and CNP, inactivating their ability to raise intracellular cGMP (PubMed:21098034). Also degrades an aberrant frameshifted 40-residue form of NPPA (fsNPPA) which is associated with familial atrial fibrillation in heterozygous patients (PubMed:21098034). Involved in antigen processing. Produces both the N terminus and the C terminus of MAGEA3-derived antigenic peptide (EVDPIGHLY) that is presented to cytotoxic T lymphocytes by MHC class I.
Cellular Location Cytoplasm, cytosol. Cell membrane {ECO:0000250|UniProtKB:P35559}. Secreted Note=Present at the cell surface of neuron cells. The membrane- associated isoform is approximately 5 kDa larger than the known cytosolic isoform
Tissue Location Detected in brain and in cerebrospinal fluid (at protein level).
Research Areas
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$ 370.00
Cat# ABO13269
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