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Anti-Fas/Cd95 Rabbit Monoclonal Antibody

     
  •  - Anti-Fas/Cd95 Rabbit Monoclonal Antibody ABO13267
    All lanes use the Antibody at 1:5K dilution for 1 hour at room temperature.
    detail
  •  - Anti-Fas/Cd95 Rabbit Monoclonal Antibody ABO13267
    Immunofluorescent analysis using the Antibody at 1:50 dilution.
    detail
  •  - Anti-Fas/Cd95 Rabbit Monoclonal Antibody ABO13267
    Immunofluorescent analysis using the Antibody at 1:50 dilution.
    detail
  •  - Anti-Fas/Cd95 Rabbit Monoclonal Antibody ABO13267
    Immunofluorescent analysis using the Antibody at 1:150 dilution.
    detail
  •  - Anti-Fas/Cd95 Rabbit Monoclonal Antibody ABO13267
    Western blot analysis of Fas expression in Ramos cell lysate.
    detail
  • SPECIFICATION
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, IHC, IF, ICC, FC
Primary Accession P25445
Host Rabbit
Isotype Rabbit IgG
Reactivity Human
Clonality Monoclonal
Format Liquid
Description Anti-Fas/Cd95 Rabbit Monoclonal Antibody . Tested in WB, IHC, ICC/IF, Flow Cytometry applications. This antibody reacts with Human.
Additional Information
Gene ID 355
Other Names Tumor necrosis factor receptor superfamily member 6, Apo-1 antigen, Apoptosis-mediating surface antigen FAS, FASLG receptor, CD95, FAS, APT1, FAS1, TNFRSF6
Calculated MW 37732 MW KDa
Application Details WB 1:500-1:2000
IHC 1:50-1:200
ICC/IF 1:50-1:200
FC 1:50
Subcellular Localization Isoform 1: Cell membrane; Single-pass type I membrane protein.
Tissue Specificity Isoform 1 and isoform 6 are expressed at equal levels in resting peripheral blood mononuclear cells. After activation there is an increase in isoform 1 and decrease in the levels of isoform 6..
Contents Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clone Names Clone: IID-6
Immunogen A synthesized peptide derived from human Fas
Purification Affinity-chromatography
Storage Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Protein Information
Name FAS
Synonyms APT1, FAS1, TNFRSF6
Function Receptor for TNFSF6/FASLG. The adapter molecule FADD recruits caspase CASP8 to the activated receptor. The resulting death-inducing signaling complex (DISC) performs CASP8 proteolytic activation which initiates the subsequent cascade of caspases (aspartate-specific cysteine proteases) mediating apoptosis. FAS-mediated apoptosis may have a role in the induction of peripheral tolerance, in the antigen- stimulated suicide of mature T-cells, or both. The secreted isoforms 2 to 6 block apoptosis (in vitro).
Cellular Location [Isoform 1]: Cell membrane; Single-pass type I membrane protein. Membrane raft [Isoform 3]: Secreted. [Isoform 5]: Secreted.
Tissue Location Isoform 1 and isoform 6 are expressed at equal levels in resting peripheral blood mononuclear cells. After activation there is an increase in isoform 1 and decrease in the levels of isoform 6.
Research Areas
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$ 370.00
Cat# ABO13267
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