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Anti-Bak BAK1 Rabbit Monoclonal Antibody

     
  •  - Anti-Bak BAK1 Rabbit Monoclonal Antibody ABO13240
    Immunohistochemical analysis of paraffin-embedded mouse liver, using Bak Antibody.
    detail
  •  - Anti-Bak BAK1 Rabbit Monoclonal Antibody ABO13240
    Figure 1. Western blot analysis of Bak using anti-Bak antibody (M01163).
    Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
    Lane 1: human Hela whole cell lysates,
    Lane 2: human 293T whole cell lysates,
    Lane 3: human Jurkat whole cell lysates,
    Lane 4: human Daudi whole cell lysates.
    After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Bak antigen affinity purified monoclonal antibody (Catalog # M01163) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:1000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Bak at approximately 23-25 kDa. The expected band size for Bak is at 23 kDa.
    detail
  •  - Anti-Bak BAK1 Rabbit Monoclonal Antibody ABO13240
    Immunofluorescent analysis of Hela cells, using Bak Antibody.
    detail
  •  - Anti-Bak BAK1 Rabbit Monoclonal Antibody ABO13240
    All lanes use the Antibody at 1:3K dilution for 1 hour at room temperature.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, IHC, IF, ICC, IP, FC
Primary Accession Q16611
Host Rabbit
Isotype Rabbit IgG
Reactivity Human, Mouse
Clonality Monoclonal
Format Liquid
Description Anti-Bak BAK1 Rabbit Monoclonal Antibody . Tested in WB, IHC, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human, Mouse.
Additional Information
Gene ID 578
Other Names Bcl-2 homologous antagonist/killer, Apoptosis regulator BAK, Bcl-2-like protein 7, Bcl2-L-7, BAK1, BAK, BCL2L7, CDN1
Calculated MW 23409 MW KDa
Application Details WB 1:500-1:2000
IHC 1:50-1:200
ICC/IF 1:50-1:200
IP 1:50
FC 1:50
Subcellular Localization Mitochondrion membrane ; Single-pass membrane protein.
Tissue Specificity Expressed in a wide variety of tissues, with highest levels in the heart and skeletal muscle.
Contents Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clone Names Clone: CHI-2
Immunogen A synthesized peptide derived from human Bak
Purification Affinity-chromatography
Storage Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Protein Information
Name BAK1
Synonyms BAK, BCL2L7, CDN1
Function Plays a role in the mitochondrial apoptotic process. Upon arrival of cell death signals, promotes mitochondrial outer membrane (MOM) permeabilization by oligomerizing to form pores within the MOM. This releases apoptogenic factors into the cytosol, including cytochrome c, promoting the activation of caspase 9 which in turn processes and activates the effector caspases.
Cellular Location Mitochondrion outer membrane; Single-pass membrane protein
Tissue Location Expressed in a wide variety of tissues, with highest levels in the heart and skeletal muscle
Research Areas
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$ 370.00
Cat# ABO13240
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