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Anti-Phospho-Paxillin (Y118) PXN Rabbit Monoclonal Antibody

     
  •  - Anti-Phospho-Paxillin (Y118) PXN Rabbit Monoclonal Antibody ABO13185
    Western blot analysis of Phospho-Paxillin (Y118) expression in (1) HeLa cell lysate; (2) HeLa cell treated with pervanadate.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB
Primary Accession P49023
Host Rabbit
Isotype Rabbit IgG
Reactivity Rat, Human, Mouse
Clonality Monoclonal
Format Liquid
Description Anti-Phospho-Paxillin (Y118) PXN Rabbit Monoclonal Antibody . Tested in WB application. This antibody reacts with Human, Mouse, Rat.
Additional Information
Gene ID 5829
Other Names Paxillin, PXN
Calculated MW 64505 MW KDa
Application Details WB 1:1000-1:2000
Subcellular Localization Cytoplasm, cytoskeleton. Cell junction, focal adhesion. Cytoplasm, cell cortex. Colocalizes with integrins at the cell periphery..
Contents Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clone Names Clone: HEG-16
Immunogen A synthesized peptide derived from human Paxillin
Purification Affinity-chromatography
Storage Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Protein Information
Name PXN
Function Cytoskeletal protein involved in actin-membrane attachment at sites of cell adhesion to the extracellular matrix (focal adhesion). Recruits other proteins such as TRIM15 to focal adhesion.
Cellular Location Cytoplasm, cytoskeleton. Cell junction, focal adhesion. Cytoplasm, cell cortex {ECO:0000250|UniProtKB:Q8VI36}. Note=Colocalizes with integrins at the cell periphery. Colocalize with PXN to membrane ruffles and the leading edge of migrating cells (PubMed:23128389). {ECO:0000250, ECO:0000269|PubMed:23128389}
Research Areas
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$ 370.00
Cat# ABO13185
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