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Anti-Phospho-IKB alpha (S32) NFKBIA Rabbit Monoclonal Antibody

     
  •  - Anti-Phospho-IKB alpha (S32) NFKBIA Rabbit Monoclonal Antibody ABO13181
    All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.
    detail
  •  - Anti-Phospho-IKB alpha (S32) NFKBIA Rabbit Monoclonal Antibody ABO13181
    Western blot analysis of Phospho-IKB alpha (S32) expression in HeLa cell lysate treated with Calyculin A and TNF-a.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, IP
Primary Accession P25963
Host Rabbit
Isotype Rabbit IgG
Reactivity Human
Clonality Monoclonal
Format Liquid
Description Anti-Phospho-IKB alpha (S32) NFKBIA Rabbit Monoclonal Antibody . Tested in WB, IP applications. This antibody reacts with Human.
Additional Information
Gene ID 4792
Other Names NF-kappa-B inhibitor alpha, I-kappa-B-alpha, IkB-alpha, IkappaBalpha, Major histocompatibility complex enhancer-binding protein MAD3, NFKBIA, IKBA, MAD3, NFKBI
Calculated MW 35609 MW KDa
Application Details WB 1:500-1:2000
IP 1:50
Subcellular Localization Cytoplasm. Nucleus. Shuttles between the nucleus and the cytoplasm by a nuclear localization signal (NLS) and a CRM1-dependent nuclear export..
Contents Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clone Names Clone: EDG-14
Immunogen A synthesized peptide derived from human Phospho-IKB alpha (S32)
Purification Affinity-chromatography
Storage Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Protein Information
Name NFKBIA
Synonyms IKBA, MAD3, NFKBI
Function Inhibits the activity of dimeric NF-kappa-B/REL complexes by trapping REL (RELA/p65 and NFKB1/p50) dimers in the cytoplasm by masking their nuclear localization signals (PubMed:1493333, PubMed:36651806, PubMed:7479976). On cellular stimulation by immune and pro-inflammatory responses, becomes phosphorylated promoting ubiquitination and degradation, enabling the dimeric RELA to translocate to the nucleus and activate transcription (PubMed:7479976, PubMed:7628694, PubMed:7796813, PubMed:7878466).
Cellular Location Cytoplasm. Nucleus. Note=Shuttles between the nucleus and the cytoplasm by a nuclear localization signal (NLS) and a CRM1-dependent nuclear export.
Research Areas
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$ 370.00
Cat# ABO13181
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