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Anti-Phospho-Stat5 (Y694) STAT5A Rabbit Monoclonal Antibody

     
  •  - Anti-Phospho-Stat5 (Y694) STAT5A Rabbit Monoclonal Antibody ABO13163
    All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.
    detail
  •  - Anti-Phospho-Stat5 (Y694) STAT5A Rabbit Monoclonal Antibody ABO13163
    Immunohistochemical analysis of paraffin-embedded human thyroid, using Phospho-Stat5 (Y694) Antibody.
    detail
  •  - Anti-Phospho-Stat5 (Y694) STAT5A Rabbit Monoclonal Antibody ABO13163
    Figure 1. Western blot analysis of STAT5A using anti-STAT5A antibody (P01087-1).
    Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
    Lane 1: human K562 whole cell lysates.
    After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-STAT5A antigen affinity purified monoclonal antibody (Catalog # P01087-1) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for STAT5A at approximately 95 kDa. The expected band size for STAT5A is at 91 kDa.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, IHC
Primary Accession P42229
Host Rabbit
Isotype Rabbit IgG
Reactivity Human, Mouse
Clonality Monoclonal
Format Liquid
Description Anti-Phospho-Stat5 (Y694) STAT5A Rabbit Monoclonal Antibody . Tested in WB, IHC applications. This antibody reacts with Human, Mouse.
Additional Information
Gene ID 6776
Other Names Signal transducer and activator of transcription 5A, STAT5A, STAT5
Calculated MW 90647 MW KDa
Application Details WB 1:500-1:2000
IHC 1:50-1:200
Subcellular Localization Cytoplasm. Nucleus. Translocated into the nucleus in response to phosphorylation.
Contents Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clone Names Clone: EDD-19
Immunogen A synthesized peptide derived from human Phospho-Stat5 (Y694)
Purification Affinity-chromatography
Storage Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Protein Information
Name STAT5A
Synonyms STAT5
Function Carries out a dual function: signal transduction and activation of transcription. Mediates cellular responses to the cytokine KITLG/SCF and other growth factors. Mediates cellular responses to ERBB4. May mediate cellular responses to activated FGFR1, FGFR2, FGFR3 and FGFR4. Binds to the GAS element and activates PRL- induced transcription. Regulates the expression of milk proteins during lactation.
Cellular Location Cytoplasm. Nucleus. Note=Translocated into the nucleus in response to phosphorylation
Research Areas
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$ 370.00
Cat# ABO13163
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