Anti-TCP1 epsilon Picoband Antibody
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application
| WB, IHC-P, ICC |
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Primary Accession | P48643 |
Host | Rabbit |
Reactivity | Human, Mouse, Rat |
Clonality | Polyclonal |
Format | Lyophilized |
Description | Rabbit IgG polyclonal antibody for T-complex protein 1 subunit epsilon(CCT5) detection. Tested with WB, IHC-P, ICC in Human;Mouse;Rat. |
Reconstitution | Add 0.2ml of distilled water will yield a concentration of 500ug/ml. |
Gene ID | 22948 |
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Other Names | T-complex protein 1 subunit epsilon, TCP-1-epsilon, CCT-epsilon, CCT5, CCTE, KIAA0098 |
Calculated MW | 59671 MW KDa |
Application Details | Immunohistochemistry(Paraffin-embedded Section), 0.5-1 µg/ml, Human, Mouse, Rat, By Heat Western blot, 0.1-0.5 µg/ml, Human, Rat Immunocytochemistry, 0.5-1 µg/ml, Human |
Subcellular Localization | Cytoplasm . Cytoplasm, cytoskeleton, microtubule organizing center, centrosome . |
Protein Name | T-complex protein 1 subunit epsilon |
Contents | Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3. |
Immunogen | E. coli-derived human TCP1 epsilon recombinant protein (Position: L443-K535). Human TCP1 epsilon shares 95.7% amino acid (aa) sequence identity with both mouse and rat TCP1 epsilon. |
Purification | Immunogen affinity purified. |
Cross Reactivity | No cross reactivity with other proteins |
Storage | At -20˚C for one year. After r˚Constitution, at 4˚C for one month. It˚Can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing. |
Name | CCT5 |
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Synonyms | CCTE, KIAA0098 {ECO:0000303|PubMed:77885 |
Function | Component of the chaperonin-containing T-complex (TRiC), a molecular chaperone complex that assists the folding of proteins upon ATP hydrolysis (PubMed:25467444). The TRiC complex mediates the folding of WRAP53/TCAB1, thereby regulating telomere maintenance (PubMed:25467444). As part of the TRiC complex may play a role in the assembly of BBSome, a complex involved in ciliogenesis regulating transports vesicles to the cilia (PubMed:20080638). The TRiC complex plays a role in the folding of actin and tubulin (Probable). |
Cellular Location | Cytoplasm. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome |
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Provided below are standard protocols that you may find useful for product applications.
Background
CCT5 is a molecular chaperone that is a member of the chaperonin containing TCP1 complex (CCT), also known as the TCP1 ring complex (TRiC). This complex consists of two identical stacked rings, each containing eight different proteins. Unfolded polypeptides enter the central cavity of the complex and are folded in an ATP-dependent manner. The complex folds various proteins, including actin and tubulin. Mutations in this gene cause hereditary sensory and autonomic neuropathy with spastic paraplegia (HSNSP). Alternative splicing results in multiple transcript variants. Related pseudogenes have been identified on chromosomes 5 and 13.
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