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Anti-Flotillin 2 Picoband Antibody

     
  • WB - Anti-Flotillin 2 Picoband Antibody ABO12309
    Anti- Flotillin 2 Picoband antibody, ABO12309, Western blottingAll lanes: Anti Flotillin 2 (ABO12309) at 0.5ug/mlLane 1: Rat Liver Tissue Lysate at 50ugLane 2: Rat Lung Tissue Lysate at 50ugLane 3: Mouse Liver Tissue Lysate at 50ugLane 4: Mouse Lung Tissue Lysate at 50ugLane 5: Mouse Testis Tissue Lysate at 50ugLane 6: Human Placenta Tissue Lysate at 50ugLane 7: A375 Whole Cell Lysate at 40ugLane 8: HELA Whole Cell Lysate at 40ugLane 9: 22RV1 Whole Cell Lysate at 40ugLane 10: NIH3T3 Whole Cell Lysate at 40ugPredicted bind size: 47KDObserved bind size: 47KD
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  • IHC - Anti-Flotillin 2 Picoband Antibody ABO12309
    Anti- Flotillin 2 Picoband antibody, ABO12309, IHC(P)IHC(P): Mouse Intestine Tissue
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  • IHC - Anti-Flotillin 2 Picoband Antibody ABO12309
    Anti- Flotillin 2 Picoband antibody, ABO12309, IHC(P)IHC(P): Rat Kidney Tissue
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  • IHC - Anti-Flotillin 2 Picoband Antibody ABO12309
    Anti- Flotillin 2 Picoband antibody, ABO12309, IHC(P)IHC(P): Human Mammary Cancer Tissue
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  • IHC - Anti-Flotillin 2 Picoband Antibody ABO12309
    Figure 5. IHC analysis of Flotillin 2 using anti-Flotillin 2 antibody (ABO12309).Flotillin 2 was detected in frozen section of mouse kidney tissue . Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-Flotillin 2 Antibody (ABO12309) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
    detail
  • IHC - Anti-Flotillin 2 Picoband Antibody ABO12309
    Figure 6. IHC analysis of Flotillin 2 using anti-Flotillin 2 antibody (ABO12309).Flotillin 2 was detected in frozen section of rat kidney tissue . Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-Flotillin 2 Antibody (ABO12309) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
    detail
  • IHC - Anti-Flotillin 2 Picoband Antibody ABO12309
    Figure 7. IHC analysis of Flotillin 2 using anti-Flotillin 2 antibody (ABO12309).Flotillin 2 was detected in frozen section of rat small intestine tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-Flotillin 2 Antibody (ABO12309) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, IHC-F
Primary Accession Q14254
Host Rabbit
Reactivity Human, Mouse, Rat
Clonality Polyclonal
Format Lyophilized
Description Rabbit IgG polyclonal antibody for Flotillin-2(FLOT2) detection. Tested with WB, IHC-P, IHC-F in Human;Mouse;Rat.
Reconstitution Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Additional Information
Gene ID 2319
Other Names Flotillin-2, Epidermal surface antigen, ESA, Membrane component chromosome 17 surface marker 1, FLOT2, ESA1, M17S1
Calculated MW 47064 MW KDa
Application Details Immunohistochemistry(Paraffin-embedded Section), 0.5-1 µg/ml, By Heat
Immunohistochemistry(Frozen Section), 0.5-1 µg/ml

Western blot, 0.1-0.5 µg/ml
Subcellular Localization Cell membrane ; Peripheral membrane protein . Membrane, caveola ; Peripheral membrane protein . Endosome . Membrane-associated protein of caveolae.
Tissue Specificity In skin, expressed in epidermis and epidermal appendages but not in dermis. Expressed in all layers of the epidermis except the basal layer. In hair follicles, expressed in the suprabasal layer but not the basal layer. Also expressed in melanoma and carcinoma cell lines, fibroblasts and foreskin melanocytes.
Protein Name Flotillin-2
Contents Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Immunogen E.coli-derived human Flotillin 2 recombinant protein (Position: K169-K344). Human Flotillin 2 shares 100% amino acid (aa) sequence identity with both mouse and rat Flotillin 2.
Purification Immunogen affinity purified.
Cross Reactivity No cross reactivity with other proteins
Storage At -20˚C for one year. After r˚Constitution, at 4˚C for one month. It˚Can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.
Protein Information
Name FLOT2
Synonyms ESA1, M17S1
Function May act as a scaffolding protein within caveolar membranes, functionally participating in formation of caveolae or caveolae-like vesicles. May be involved in epidermal cell adhesion and epidermal structure and function.
Cellular Location Cell membrane; Peripheral membrane protein. Membrane, caveola; Peripheral membrane protein. Endosome Membrane; Lipid-anchor. Note=Membrane- associated protein of caveolae
Tissue Location In skin, expressed in epidermis and epidermal appendages but not in dermis. Expressed in all layers of the epidermis except the basal layer. In hair follicles, expressed in the suprabasal layer but not the basal layer. Also expressed in melanoma and carcinoma cell lines, fibroblasts and foreskin melanocytes
Research Areas
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Background

FLOT2 (Flotillin 2), also known as ESA1 or M17S1, is a protein that in humans is encoded by the FLOT2 gene. Schroeder et al. (1991) isolated a cDNA for an epidermal surface antigen believed to be involved in epidermal cell adhesion. By analysis of a somatic cell hybrid panel and in situ hybridization using the ESA cDNA, the gene was mapped to 17q11-q12 in the region containing the NF1 gene. Bickel et al. (1997) found that mouse Flot2 consistently copurifies with Flot1 and with caveolin-1 in the purification of caveolin-rich membranes. Using a quantitative proteomic analysis of cultured mouse neuronal stem cells, Li et al. (2012) found that palmitoylation and oligomerization of flotillin-2 was abolished in homozygous Dhhc5 mutant neuronal stem cells. The absolute amount of flotillin-2 was not changed in Dhhc5 mutant neurons.

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$ 370.00
Cat# ABO12309
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