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Anti-EBAG9 Picoband Antibody

     
  • WB - Anti-EBAG9 Picoband Antibody ABO12240
    Anti- EBAG9 Picoband antibody, ABO12240, Western blottingAll lanes: Anti EBAG9 (ABO12240) at 0.5ug/mlLane 1: Rat Testis Tissue Lysate at 50ugLane 2: 22RV1 Whole Cell Lysate at 40ugLane 3: HELA Whole Cell Lysate at 40ugLane 4: MCF-7 Whole Cell Lysate at 40ugLane 5: JURKAT Whole Cell Lysate at 40ugPredicted bind size: 34KDObserved bind size: 34KD
    detail
  • IHC - Anti-EBAG9 Picoband Antibody ABO12240
    Anti- EBAG9 Picoband antibody, ABO12240,IHC(P)IHC(P): Mouse Testis Tissue
    detail
  • IHC - Anti-EBAG9 Picoband Antibody ABO12240
    Anti- EBAG9 Picoband antibody, ABO12240,IHC(P)IHC(P): Rat Testis Tissue
    detail
  • IHC - Anti-EBAG9 Picoband Antibody ABO12240
    Anti- EBAG9 Picoband antibody, ABO12240,IHC(P)IHC(P): Human Prostatic Cancer Tissue
    detail
  • SPECIFICATION
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P
Primary Accession O00559
Host Rabbit
Reactivity Human, Mouse, Rat
Clonality Polyclonal
Format Lyophilized
Description Rabbit IgG polyclonal antibody for Receptor-binding cancer antigen expressed on SiSo cells(EBAG9) detection. Tested with WB, IHC-P in Human;Mouse;Rat.
Reconstitution Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Additional Information
Gene ID 9166
Other Names Receptor-binding cancer antigen expressed on SiSo cells, Cancer-associated surface antigen RCAS1, Estrogen receptor-binding fragment-associated gene 9 protein, EBAG9, RCAS1
Calculated MW 24377 MW KDa
Application Details Immunohistochemistry(Paraffin-embedded Section), 0.5-1 µg/ml, Human, Mouse, Rat, By Heat
Western blot, 0.1-0.5 µg/ml, Human, Rat
Subcellular Localization Golgi apparatus membrane ; Single-pass type III membrane protein . According to PubMed:10426319, it also exists as a soluble form which has the same biological activities. The existence of such soluble form is however uncertain.
Tissue Specificity Widely expressed. Expressed in ovary, testis, prostate, thymus, muscle and heart, but not in small intestine, colon, lymph nodes, or peripherical blood lymphocytes. The protein is not detected in any of the above organs.
Protein Name Receptor-binding cancer antigen expressed on SiSo cells
Contents Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Immunogen E.coli-derived human EBAG9 recombinant protein (Position: R31-S213). Human EBAG9 shares 97.8% and 94.5% amino acid (aa) sequence identity with mouse and rat EBAG9, respectively.
Purification Immunogen affinity purified.
Cross Reactivity No cross reactivity with other proteins
Storage At -20˚C for one year. After r˚Constitution, at 4˚C for one month. It˚Can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.
Protein Information
Name EBAG9
Synonyms RCAS1
Function May participate in suppression of cell proliferation and induces apoptotic cell death through activation of interleukin-1-beta converting enzyme (ICE)-like proteases.
Cellular Location Golgi apparatus membrane; Single-pass type III membrane protein. Note=According to PubMed:10426319, it also exists as a soluble form which has the same biological activities The existence of such soluble form is however uncertain
Tissue Location Widely expressed. Expressed in ovary, testis, prostate, thymus, muscle and heart, but not in small intestine, colon, lymph nodes, or peripherical blood lymphocytes. The protein is not detected in any of the above organs
Research Areas
Citations (0)
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Background

Receptor-binding cancer antigen expressed on SiSo cells is a protein that in humans is encoded by the EBAG9 gene. This gene was identified as an estrogen-responsive gene. Regulation of transcription by estrogen is mediated by estrogen receptor, which binds to the estrogen-responsive element found in the 5'-flanking region of this gene. And the encoded protein is a tumor-associated antigen that is expressed at high frequency in a variety of cancers. Alternate splicing results in multiple transcript variants. A pseudogene of this gene has been defined on chromosome 10.

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$ 370.00
Cat# ABO12240
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