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Anti-Cdk4 Picoband Antibody

     
  • WB - Anti-Cdk4 Picoband Antibody ABO12222
    Anti- Cdk4 Picoband antibody, ABO12222, Western blottingAll lanes: Anti Cdk4 (ABO12222) at 0.5ug/mlLane 1: Rat Gaster Tissue Lysate at 50ugLane 2: Rat Thymus Tissue Lysate at 50ugLane 3: HELA Whole Cell Lysate at 40ugLane 4: A549 Whole Cell Lysate at 40ugLane 5: SKOV Whole Cell Lysate at 40ugLane 6: 22RV1 Whole Cell Lysate at 40ugPredicted bind size: 34KDObserved bind size: 34KD
    detail
  • IHC - Anti-Cdk4 Picoband Antibody ABO12222
    Anti- Cdk4 Picoband antibody, ABO12222, IHC(P)IHC(P): Mouse Intestine Tissue
    detail
  • IHC - Anti-Cdk4 Picoband Antibody ABO12222
    Anti- Cdk4 Picoband antibody, ABO12222, IHC(P)IHC(P): Rat Intestine Tissue
    detail
  • IHC - Anti-Cdk4 Picoband Antibody ABO12222
    Anti- Cdk4 Picoband antibody, ABO12222, IHC(P)IHC(P): Human Lung Cancer Tissue
    detail
Anti- Cdk4 Picoband antibody, ABO12222, Western blottingAll lanes: Anti Cdk4 (ABO12222) at 0.5ug/mlLane 1: Rat Gaster Tissue Lysate at 50ugLane 2: Rat Thymus Tissue Lysate at 50ugLane 3: HELA Whole Cell Lysate at 40ugLane 4: A549 Whole Cell Lysate at 40ugLane 5: SKOV Whole Cell Lysate at 40ugLane 6: 22RV1 Whole Cell Lysate at 40ugPredicted bind size: 34KDObserved bind size: 34KD
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P
Primary Accession P11802
Host Rabbit
Reactivity Human, Mouse, Rat
Clonality Polyclonal
Format Lyophilized
Description Rabbit IgG polyclonal antibody for Cyclin-dependent kinase 4(CDK4) detection. Tested with WB, IHC-P in Human;Mouse;Rat.
Reconstitution Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Additional Information
Gene ID 1019
Other Names Cyclin-dependent kinase 4, 2.7.11.22, Cell division protein kinase 4, PSK-J3, CDK4
Calculated MW 33730 MW KDa
Application Details Immunohistochemistry(Paraffin-embedded Section), 0.5-1 µg/ml, Human, Mouse, Rat, By Heat
Western blot, 0.1-0.5 µg/ml, Human, Rat
Subcellular Localization Cytoplasm. Nucleus. Membrane. Cytoplasmic when non-complexed. Forms a cyclin D-CDK4 complex in the cytoplasm as cells progress through G(1) phase. The complex accumulates on the nuclear membrane and enters the nucleus on transition from G(1) to S phase. Also present in nucleoli and heterochromatin lumps. Colocalizes with RB1 after release into the nucleus.
Protein Name Cyclin-dependent kinase 4
Contents Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Immunogen E.coli-derived human Cdk4 recombinant protein (Position: G201-E303). Human Cdk4 shares 94.2% amino acid (aa) sequence identity with both mouse and rat Cdk4.
Purification Immunogen affinity purified.
Cross Reactivity No cross reactivity with other proteins
Storage At -20˚C for one year. After r˚Constitution, at 4˚C for one month. It˚Can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.
Sequence Similarities Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.
Protein Information
Name CDK4
Function Ser/Thr-kinase component of cyclin D-CDK4 (DC) complexes that phosphorylate and inhibit members of the retinoblastoma (RB) protein family including RB1 and regulate the cell-cycle during G(1)/S transition. Phosphorylation of RB1 allows dissociation of the transcription factor E2F from the RB/E2F complexes and the subsequent transcription of E2F target genes which are responsible for the progression through the G(1) phase. Hypophosphorylates RB1 in early G(1) phase. Cyclin D-CDK4 complexes are major integrators of various mitogenenic and antimitogenic signals. Also phosphorylates SMAD3 in a cell-cycle-dependent manner and represses its transcriptional activity. Component of the ternary complex, cyclin D/CDK4/CDKN1B, required for nuclear translocation and activity of the cyclin D-CDK4 complex.
Cellular Location Cytoplasm. Nucleus. Nucleus membrane. Note=Cytoplasmic when non-complexed Forms a cyclin D-CDK4 complex in the cytoplasm as cells progress through G(1) phase. The complex accumulates on the nuclear membrane and enters the nucleus on transition from G(1) to S phase. Also present in nucleoli and heterochromatin lumps. Colocalizes with RB1 after release into the nucleus.
Research Areas
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$ 370.00
Cat# ABO12222
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