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Anti-ROCK2 Picoband Antibody

     
  • WB - Anti-ROCK2 Picoband Antibody ABO12078
    Anti- ROCK2 Picoband antibody, ABO12078, Western blottingAll lanes: Anti ROCK2 (ABO12078) at 0.5ug/mlLane 1: Rat Brain Tissue Lysate at 50ugLane 2: Rat Liver Tissue Lysate at 50ugLane 3: HELA Whole Cell Lysate at 40ugLane 4: CEM Whole Cell Lysate at 40ugLane 5: A549 Whole Cell Lysate at 40ugLane 6: MCF-7 Whole Cell Lysate at 40ugPredicted bind size: 161KDObserved bind size: 161KD
    detail
  • IHC - Anti-ROCK2 Picoband Antibody ABO12078
    Anti- ROCK2 Picoband antibody, ABO12078,IHC(P)IHC(P): Mouse Cardiac Muscle Tissue
    detail
  • IHC - Anti-ROCK2 Picoband Antibody ABO12078
    Anti- ROCK2 Picoband antibody, ABO12078,IHC(P)IHC(P): Rat Brain Tissue
    detail
  • IHC - Anti-ROCK2 Picoband Antibody ABO12078
    Anti- ROCK2 Picoband antibody, ABO12078,IHC(P)IHC(P): Human Mammary Cancer Tissue
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P
Primary Accession O75116
Host Rabbit
Reactivity Human, Mouse, Rat
Clonality Polyclonal
Format Lyophilized
Description Rabbit IgG polyclonal antibody for Rho-associated protein kinase 2(ROCK2) detection. Tested with WB, IHC-P in Human;Mouse;Rat.
Reconstitution Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Additional Information
Gene ID 9475
Other Names Rho-associated protein kinase 2, 2.7.11.1, Rho kinase 2, Rho-associated, coiled-coil-containing protein kinase 2, Rho-associated, coiled-coil-containing protein kinase II, ROCK-II, p164 ROCK-2, ROCK2, KIAA0619
Calculated MW 160900 MW KDa
Application Details Immunohistochemistry(Paraffin-embedded Section), 0.5-1 µg/ml, Human, Mouse, Rat, By Heat
Western blot, 0.1-0.5 µg/ml, Human, Mouse, Rat
Subcellular Localization Cytoplasm. Cell membrane ; Peripheral membrane protein . Nucleus. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Cytoplasmic, and associated with actin microfilaments and the plasma membrane. .
Protein Name Rho-associated protein kinase 2
Contents Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Immunogen E.coli-derived human ROCK2 recombinant protein (Position: E652-D923). Human ROCK2 shares 94.9% and 95.2% amino acid (aa) sequence identity with mouse and rat ROCK2, respectively.
Purification Immunogen affinity purified.
Cross Reactivity No cross reactivity with other proteins.
Storage At -20˚C for one year. After r˚Constitution, at 4˚C for one month. It˚Can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.
Sequence Similarities Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family.
Protein Information
Name ROCK2
Synonyms KIAA0619
Function Protein kinase which is a key regulator of actin cytoskeleton and cell polarity. Involved in regulation of smooth muscle contraction, actin cytoskeleton organization, stress fiber and focal adhesion formation, neurite retraction, cell adhesion and motility via phosphorylation of ADD1, BRCA2, CNN1, EZR, DPYSL2, EP300, MSN, MYL9/MLC2, NPM1, RDX, PPP1R12A and VIM. Phosphorylates SORL1 and IRF4. Acts as a negative regulator of VEGF-induced angiogenic endothelial cell activation. Positively regulates the activation of p42/MAPK1- p44/MAPK3 and of p90RSK/RPS6KA1 during myogenic differentiation. Plays an important role in the timely initiation of centrosome duplication. Inhibits keratinocyte terminal differentiation. May regulate closure of the eyelids and ventral body wall through organization of actomyosin bundles. Plays a critical role in the regulation of spine and synaptic properties in the hippocampus. Plays an important role in generating the circadian rhythm of the aortic myofilament Ca(2+) sensitivity and vascular contractility by modulating the myosin light chain phosphorylation.
Cellular Location Cytoplasm. Cell membrane; Peripheral membrane protein. Nucleus. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome Note=Cytoplasmic, and associated with actin microfilaments and the plasma membrane.
Tissue Location Expressed in the brain (at protein level).
Research Areas
Citations (0)
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Background

Rho-associated kinase (ROCK), including the ROCK-I and ROCK-II isoforms, is a protein kinase involved in signaling from Rho to actin cytoskeleton. Serine/threonine kinase ROCK II/Rho kinase, which is an isozyme of ROCK I, is one of the targets for the small GTPase Rho. ROCK II regulates the formation of actin stress fibers and focal adhesions, cytokinesis, smooth muscle contraction, and the activation of c-fos serum response element. Sequencing analysis has shown that human ROCK II contains 1388 amino acid residues with a calculated molecular mass of approximately 161 kDa. Fluorescence in situ hybridization analysis showed that the human ROCK II gene is located on chromosome 2p24. Thumkeo et al. concluded that ROCK-II is essential in inhibiting blood coagulation and maintaining blood flow in the endothelium-free labyrinth layer and that loss of ROCK-II leads to thrombus formation, placental dysfunction, intrauterine growth retardation, and fetal death.

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$ 370.00
Cat# ABO12078
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